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T0678
Anti-Tryptophan Hydroxylase Antibody
mouse monoclonal, WH-3
Synonym(e):
Anti-TPH
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100 μL
CHF 506.00
0.2 ML
CHF 629.00
CHF 506.00
Voraussichtliches Versanddatum12. Mai 2025
Für Ihr Target ist ein rekombinanter, konservierungsmittelfreier Antikörper verfügbar. Probieren Sie ZRB1305
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100 μL
CHF 506.00
0.2 ML
CHF 629.00
About This Item
CHF 506.00
Voraussichtliches Versanddatum12. Mai 2025
Für Ihr Target ist ein rekombinanter, konservierungsmittelfreier Antikörper verfügbar. Probieren Sie ZRB1305
Bulk-Bestellung anfordern
Empfohlene Produkte
Produktbezeichnung
Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, clone WH-3, ascites fluid
Biologische Quelle
mouse
Qualitätsniveau
Konjugat
unconjugated
Antikörperform
ascites fluid
Antikörper-Produkttyp
primary antibodies
Klon
WH-3, monoclonal
Mol-Gew.
antigen 55 kDa
Enthält
15 mM sodium azide
Speziesreaktivität
human, monkey, rabbit, rat
Methode(n)
immunohistochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using rabbit pineal gland extract
Isotyp
IgG3
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Lagertemp.
−20°C
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... TPH1(7166) , TPH2(121278)
rat ... Tph1(24848) , Tph2(317675)
Allgemeine Beschreibung
Monoclonal Anti-Tryptophan Hydroxylase (mouse IgG3 isotype) is derived from the WH-3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Tryptophan hydroxylase (TPH; L-tryptophan tetrahydropteridine:oxygen oxidoreductase; EC 1.14.16.4), the rate-limiting enzyme for the synthesis of serotonin, is a very specific enzyme found only inside serotoninergic neurons.
The tryptophan hydroxylase 1 gene (TPH1) is a 444 amino acid protein encoded by the gene spanning 29 kbp with11 exons on human chromosome 11p15.3-p14. The enzyme is expressed in the gut, spleen, thymus, pituitary and also in the pineal gland.
Spezifität
This antibody reacts specifically with tryptophan hydroxylase.
Immunogen
recombinant rabbit tryptophan hydroxylase.
Anwendung
Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
- immunoblot
- immunocytochemistry
Biochem./physiol. Wirkung
The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan, which is a precursor to the neurotransmitter serotonin. Variation in the gene expression affects prefrontal cortex activation during response inhibition. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders. The encoded protein plays a vital role in regulation of cardiovascular function.
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Beschreibung
Preisangaben
Lagerklassenschlüssel
10 - Combustible liquids
WGK
nwg
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Anthony C Ruocco et al.
Neuropsychology, 30(1), 18-27 (2015-12-29)
The tryptophan hydroxylase 1 gene (TPH1) catalyzes the formation of 5-hydroxytryptophan, a precursor to the neurotransmitter serotonin. Variations in the gene encoding this enzyme may underlie difficulties in impulse control; however, the proximate relationship between risk alleles for polymorphisms in
Distinct neurochemical and functional properties of GAD67-containing 5-HT neurons in the rat dorsal raphe nucleus
Shikanai H, et al.
The Journal of Neuroscience, 32(41), 14415-14426 (2012)
Brain tryptophan hydroxylase: purification of, production of antibodies to, and cellular and ultrastructural localization in serotonergic neurons of rat midbrain
Joh TH, et al.
Proceedings of the National Academy of Sciences of the USA, 72(9), 3575-3579 (1975)
Honghai Zhang et al.
Epilepsia, 57(8), 1228-1235 (2016-06-16)
The DBA/1 mouse is a relevant animal model of sudden unexpected death in epilepsy (SUDEP), as it exhibits seizure-induced respiratory arrest (S-IRA) evoked by acoustic stimulation, followed by cardiac arrhythmia and death. Defects in serotonergic neurotransmission may contribute to S-IRA.
Kadri Andre et al.
BMC psychiatry, 13, 118-118 (2013-04-20)
In major depression, one of the candidate genes possibly affecting the risk and severity of symptoms has been found to be tryptophan hydroxylase (TPH1). Variation in treatment response to antidepressive agents according to TPH1 genotype has also been found in
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What is the Department of Transportation shipping information for this product?
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Do you have an immunohistochemistry protocol for use with Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse?
1 answer-
Free floating sections (4% paraformaldehyde fixation)1. Incubate 30 minutes at room temperature in 0.3% Triton X-100, 5% normal serum.2. Incubate with the antibody diluted 1:10,000 in PBS, 0.3% Triton X-100, 3.5% normal serum and 0.05% BSA for 36-48 hours.3. Rinse 3 times for 5 minutes each in PBS.4. Add the biotinylated anti-mouse IgG3 diluted in PBS containing 0.3% Triton X-100 and 3.5% normal serum for 1 hour at room temperature.5. Rinse 3 times for 5 minutes each in PBS.6. Process with Avidin Biotin-HRP.7. Complete the reaction with a 2-10 minute incubation in PBS containing 0.05% DAB and 0.015% hydrogen peroxide (H2O2).8. Mount the sections on gel coated slides and air dry.
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Does Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, cross-react with tyrosine or phenylalanine hydroxylases?
1 answer-
This antibody, unlike some other antibodies to tryptophan hydroxylases (WH) in the market, does not cross react with tyrosine hydroxylase (TH) or with phenylalanine hydroxylase (PH). The epitope recognized by the antibody is not located in the Pterin Binding Region, which is homologous among PH, TH and WH. The specificity to WH is confirmed by the single band obtained at the 55KD region, while TH has a higher MW (60-68KD), depending on the submit) and PH has a lower MW. It has also been confirmed by collaborating experts. See Journal of Neuroscience Methods, 114(2), 205-212 (2002).
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Can Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, be used to stain mouse brain sections?
1 answer-
This antibody has been used successfully on mouse brain slices in an immunohistochemical application. The IHC results from an experiment employing T0678 at 1:500 in mouse brain slice staining clearly showed the dorsal nuclear raphe (which are the serotonergic neurons).
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