SBR00050

FITC Labeled D-Lysine

Suitable for bacterial labeling

• Synonym(e): FADL, FDAA
• Empirische Formel (Hill-System): C₂₇H₂₅N₃O₇S
• Molekulargewicht: 535.57
• UNSPSC-Code: 12352209
• NACRES: NA.24

Eigenschaften

• Qualitätsniveau: 200
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Bacterial Peptidoglycan is a polymer consisting of sugars and amino acids, a mesh-like rigid layer that forms the cell wall. D-Amino Acids (D-AAs) are metabolically incorporated onto the bacterial cell wall by D,D-transpeptidase and/or L,D-transpeptidase. It was recently discovered that those transpeptidases catalyze the metabolic incorporation of exogenous D-AAs with almost no restriction of the side-chain identity. Modification of a D-AA with molecular fluorophores provides fluorescent D-amino acids that can efficiently label in situ peptidoglycan in diverse bacterial species.

Fluorescein Labeled D-Lysine is a fluorescent derivative of D-Lysine that is obtained by a synthetic conjugation of D-Lysine to a fluorophore. Fluorescein Labeled D-Lysine has high biocompatibility and suitability for labeling peptidoglycans in live bacteria. Additionally, it can be used in tandem with other stains such as: FITC-labelled Alanine, Rhodamine B labelled Polymyxin B, FITC labelled vancomycin and Dansyl labelled polymyxin B (SBR00049, SBR00036, SBR00028, SBR00029).

Anwendung

Fluorescent labeled D-AAs can be used for many applications including:

• Bacterial cell wall morphology
• Bacterial cell wall formation or remodeling activity
• Bacterial viability/activity
• Identify bacterial activity on surfaces or in substances
• Differentiation betweenvarious bacterial strains according to their incorporation profile of differentD amino acids and sugars
  

Hinweis zur Analyse

• Fluorescent microscopy application: Fluorescein Labeled D-Lysine has excitation/emission wavelength range at 495 nm/515 nm.
• The recommended working concentration in fluorescent microscopy imaging application is between 250μM-500 μM in DMSO.
• Aliquots of the DMSO solution can be stored at -20°C, protected from light for at least one month.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable