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SAB2702197

Sigma-Aldrich

Monoclonal Anti-GFP antibody produced in mouse

clone GT859, affinity isolated antibody

Synonym(e):

Mouse Monoclonal Anti-Green Fluorescent Protein

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

100

Konjugat

unconjugated

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

GT859, monoclonal

Form

buffered aqueous solution

Speziesreaktivität

mouse

Konzentration

1mg/mL

Methode(n)

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1000-10000

Isotyp

IgG2b

Versandbedingung

wet ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

Green fluorescent protein (GFP) is a fluorescent protein. It is mainly isolated from the luminous organ of the jellyfish Aequorea victoria.

Immunogen

Full length GFP recombinant protein

Anwendung

Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, ICC/IF: 1:100-1:2000, IHC-Fr: Assay-dependent dilution, IHC-P: Assay-dependent dilution, IP: 1:100-1:500, WB: 1:1000-1:10000, IHC-Wm: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Monoclonal Anti-GFP antibody has been used in gait analysis.

Biochem./physiol. Wirkung

Green fluorescent protein (GFP) is used as a marker for gene expression. It is also used as a tag to study about protein localization in several organisms. GFP can form the intrinsic chromophore without the help of cofactors or enzymatic components.

Leistungsmerkmale und Vorteile

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Sonstige Hinweise

Purification: Affinity purified by Protein G

Physikalische Form

Phosphate-buffered saline, no preservative added.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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FACS-optimized mutants of the green fluorescent protein (GFP)
Cormack B P, et al.
Gene, 173(1), 33-38 (1996)
Fluorescence-based biosensors: from concepts to applications
Progress in Molecular Biology and Translational Science, 113(1) (2012)
Yang Yang et al.
Nature neuroscience, 19(10), 1348-1355 (2016-09-07)
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Kai Lǚ et al.
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A growing number of evidence shows that some invertebrates possess an antiviral immunity parallel to the interferon (IFN) system of higher vertebrates. For example, the IRF (interferon regulatory factor)-Vago-JAK/STAT regulatory axis in an arthropod, shrimp Litopenaeus vannamei (whiteleg shrimp) is
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