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S9396

Sigma-Aldrich

Sphingomyelinase from Bacillus cereus

buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

Synonym(e):

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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CHF 339.00
50 UNITS
CHF 409.00

CHF 339.00

Voraussichtliches Versanddatum01. Juni 2025

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CHF 339.00
50 UNITS
CHF 409.00

About This Item

CAS-Nummer:
9031-54-3
EC-Nummer:
3.1.4.12 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00132362
UNSPSC-Code:
12352204
NACRES:
NA.54

CHF 339.00

Voraussichtliches Versanddatum01. Juni 2025

Bulk-Bestellung anfordern

Form

buffered aqueous glycerol solution

Qualitätsniveau

200

Spezifische Aktivität

≥100 units/mg protein (Lowry)

Mol-Gew.

34.2 kDa

Fremdaktivität

Phospholipase C ≤1%

Lagertemp.

2-8°C

Angaben zum Gen

Bacillus cereus ATCC 10987 ... spH(2751530)

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Allgemeine Beschreibung

Sphingomyelinase (SMase) is a hydrolase,[1] of 34.2 kDa and its active site contain as a minimum of two magnesium atoms.[2]

Anwendung

Sphingomyelinase from Bacillus cereus has been used:
  • in the study to analyse the effect of sphingomyelinase on Rubella virus infectivity in cell lines[3]
  • in lipoprotein modification for the generation of cholesterol crystals in vitro[4]
  • to aggregate LDL (low-density lipoprotein)[5]

Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. [6] Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems. [7]

Biochem./physiol. Wirkung

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.
Sphingomyelin phosphodiesterase (SMPD) participates in the metabolic reactions comprising sphingomyelin.[1] SMPD has the ability to prevent colorectal carcinoma and colitis.[1]

Einheitendefinition

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

Physikalische Form

Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000400206
0000371482
0000400206
0000371482
0000237638

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Die Dokumentenbibliothek aufrufen

Extracellular lipids accumulate in human carotid arteries as distinct three-dimensional structures and have proinflammatory properties
Lehti S, et al.
The American Journal of Pathology, 188(2), 525-538 (2018)
The Bacillus cereus group
Molecular Medical Microbiology, 1041-1078 (2015)
G Pabst et al.
European biophysics journal : EBJ, 41(10), 915-929 (2012-06-05)
Biological membranes can be targets for compounds that either disrupt their barrier function or affect protein function via membrane-mediated processes. Biophysical studies on membrane-mimetic systems composed of membrane lipids have contributed substantially to our knowledge on the pertaining membrane physics
Analysis of the Effect of Sphingomyelinase on Rubella Virus Infectivity in Two Cell Lines
Otsuki N, et al.
Journal of virology (2017)
Antioxidants inhibit low density lipoprotein oxidation less at lysosomal pH: A possible explanation as to why the clinical trials of antioxidants might have failed
Ahmad F and Leake D S
Chemistry and Physics of Lipids, 213, 13-24 (2018)
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Questions

1–2 of 2 Questions  

  1. what is the concentration and volumn of this product s9396-25units

    1 answer

    1. The concentration and volume of this product vary from lot to lot. The protein content and activity of this enzyme are reported on the lot specific Certificate of Analysis. The volume may be calculated using this information. For example, a given lot that reports a protein content of 1 mg/mL and an activity of 300 units per milligram protein, will have a volume of 167 uL. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/US/en/product/sigma/s9396#product-documentation

      Please see the following link to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/230/s9396dat.pdf

      Helpful?

  2. Can you tell me concentration according to volume?????? Cat No: S9396-50UN

    1 answer

    1. Prot. Content (mg Protein/ml) and Enzym. Assay (Activity U/ml) are reported in batch specific Certificate of Analysis . Please see the link below to access a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/s9396#product-documentation

      Helpful?

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