S9396

Sphingomyelinase from Bacillus cereus

buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

• Synonym(e): Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase
• CAS-Nummer: 9031-54-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 3.1.4.12 (BRENDA, IUBMB)
• MDL number: MFCD00132362
• Specific activity: ≥100 units/mg protein (Lowry)

Eigenschaften

• Quality Segment: 200
• form: buffered aqueous glycerol solution
• specific activity: ≥100 units/mg protein (Lowry)
• mol wt: 34.2 kDa
• foreign activity: Phospholipase C ≤1%
• storage temp.: 2-8°C
• Gene Information: Bacillus cereus ATCC 10987 ... spH(2751530)

Beschreibung

General description

Sphingomyelinase (SMase) is a hydrolase, of 34.2 kDa and its active site contain as a minimum of two magnesium atoms.

Application

Sphingomyelinase from Bacillus cereus has been used:

• in the study to analyse the effect of sphingomyelinase on Rubella virus infectivity in cell lines
• in lipoprotein modification for the generation of cholesterol crystals in vitro
• to aggregate LDL (low-density lipoprotein)

Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. [1] Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems. [2]

Biochem/physiol Actions

Sphingomyelin phosphodiesterase (SMPD) participates in the metabolic reactions comprising sphingomyelin. SMPD has the ability to prevent colorectal carcinoma and colitis.

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Physical form

Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5.

Other Notes

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable