R6267

RANTES human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(e): Regulated on Activation, Normal T-cell Expressed and Secreted
• EG-Nummer: 200-835-2
• MDL-Nummer: MFCD00213042
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: 1-5 ng/mL EC₅₀
• Mol-Gew.: 7.8-8.7 kDa
• Verpackung: pkg of 10 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: endotoxin, tested
• UniProt-Hinterlegungsnummer: P13501
• Lagertemp.: −20°C
• Angaben zum Gen: human ... CCL5(6352)

Beschreibung

Allgemeine Beschreibung

Regulated on activation, normal T cell expressed and secreted (RANTES), also known as C-C motif chemokine ligand 5 (CCL5), is encoded by the gene mapped to human chromosome 17. RANTES belongs to the chemokine family of proinflammatory basic polypeptides and is expressed by various hematopoietic and non-hematopoietic cell types involved in homing and migration of effector and memory T cells during acute infections.

Biochem./physiol. Wirkung

Regulated on activation, normal T cell expressed and secreted (RANTES) is a chemoattractant towards monocytes, dendritic cells (DCs), memory T cells (CD4⁺/CD45RO), basophils, and eosinophils. The encoded chemokine protein functions as a ligand for CCR1, CCR3, and CCR5. RANTES inhibits the action of R5 strains of HIV-1 via blocking CCR5 receptor. A variant of RANTES gene is linked with susceptibility to HIV-1 infection and disease progression. Hence, this can be used as a potent biomarker to determine the prognosis of HIV -1 infection.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

Hinweis zur Analyse

The biological activity is tested in culture using Baf/3 cells. Chemotaxis: EC₅₀: 1-5 ng/mL

Sicherheitshinweise

• Piktogramme: GHS02,GHS07
• Signalwort: Danger
• H-Sätze: H225,H302 + H312 + H332,H319
• P-Sätze: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2
• Lagerklassenschlüssel: 3 - Flammable liquids
• WGK: WGK 2
• Flammpunkt (°F): 35.6 °F
• Flammpunkt (°C): 2 °C