Product P1944 - Phenol:Chloroform: Iso-amyl alcohol (125:24:1) is acidic. As such, the RNA isolates in an acidic pH and will partition the genomic DNA into the organic phase leaving only the RNA in the top aqueous phase.
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Wichtige Dokumente
P1944
Phenol:Chloroform:Iso-amyl alcohol (125:24:1)
for molecular biology
Synonym(e):
phenol chloroform RNA extraction
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About This Item
UNSPSC-Code:
12190000
NACRES:
NA.52
Qualität:
for molecular biology
Assay:
≥99.0%
Anwendung(en):
life science and biopharma
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Empfohlene Produkte
Qualität
for molecular biology
Qualitätsniveau
Assay
≥99.0%
Form
liquid
pH-Wert
4.3-4.7
Anwendung(en)
life science and biopharma
Lagertemp.
2-8°C
Allgemeine Beschreibung
Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.[1]
Anwendung
Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:
Signalwort
Danger
Gefahreneinstufungen
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2
Zielorgane
Liver,Kidney, Nervous system,Kidney,Liver,Skin
Lagerklassenschlüssel
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flammpunkt (°F)
175.0 °F - closed cup
Flammpunkt (°C)
79.44 °C - closed cup
Persönliche Schutzausrüstung
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Artikel
Understand how mRNA vaccines induce immunity. and read how synthetic mRNA is prepared for vaccine immunogens and other biopharmaceuticals. Find reagents for synthesis of mRNA.
Erfahren Sie, wie mRNA-Impfstoffe Immunität induzieren und wie synthetische mRNA für Impfstoffe und andere Biopharmazeutika hergestellt wird. Entdecken Sie Reagenzien für die Synthese von mRNA.
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Hello, Your product is fractionated into two phases. Should I use lower phase for RNA extraction? Thank you
1 answer-
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