MSP01
Membran-Gerüstprotein 1D1
recombinant, expressed in E. coli, buffered aqueous solution
Synonym(e):
MSP1D1, MSP1T2
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(4)
About This Item
UNSPSC-Code:
12352200
NACRES:
NA.26
Empfohlene Produkte
Rekombinant
expressed in E. coli
Assay
≥90% (SDS-GE)
Form
buffered aqueous solution
Mol-Gew.
24,661.6 Da
Löslichkeit
water: soluble
Versandbedingung
ambient
Lagertemp.
−20°C
Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich
Allgemeine Beschreibung
Research area: Cell signaling. Nanodiscs are non-covalent structures that contain a phospholipidbilayer and a membrane scaffold protein (MSP), a genetically engineeredprotein, that mimics the function of Apolipoprotein A-1 (ApoA-1). The firstMSP, MSP1, was engineered with its sequence based on the sequence of A-1, butwithout the globular N-terminal domain of native A-1. The Membrane ScaffoldProtein 1D1 (MSP1D1) variant of MSP1 deletes the first 11 amino acids in theHelix 1 portion (referred to as “H0.5” in the accompanying figure) of theoriginal MSP1 sequence.It consists of an N-terminal histidine-tagged protein with a tobaccoetch virus (TEV) protease cleavage site that is present between the proteinsequence and histidine-tag.Nano-disctechnology is an approach that allows aqueous soluble membrane proteins in anative-like bilayer environment that enables the membrane proteins to remainactive and stable.
Anwendung
Das Nanodisc-System wird zum Integrieren einer Vielzahl von Proteinen, einschließlich GPCRs, P450s, Bacteriorhodopsin, Gerinnungsfaktoren, Choleratoxin, TAR-Rezeptor und Aromatase, eingesetzt.
Nanodiscs mit löslichen Lipiddoppelschichten werden in vielen Anwendungen eingesetzt, um Membranproteine in wässrigen Lösungen in einer naturgetreuen Doppelschicht, in der sie ihre Monodispersität und Aktivität aufrechterhalten, löslich zu machen. Die wichtigste Komponente der Nanodiscs ist der umschließende amphipathische helikale Proteingürtel (Membran-Gerüstprotein).
For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.
Biochem./physiol. Wirkung
MSP1D1 can self-assembleinto discoidal nanoparticles known as nano-discs. These nano-discs may be used inthe field of medicine, biotechnology, and membrane protein studies.
Zur Herstellung von Nanodiscs mit einem Durchmesser von ~9,7 nm
Rechtliche Hinweise
Die Nanodisc-Technologie und viele Anwendungen davon sind unter den folgenden Patenten der University of Illinois geschützt.
- 7,691,414 Membrane scaffold proteins
- 7,662,410 Membrane scaffold proteins and embedded membrane proteins
- 7,622,437 Tissue factor compositions and methods
- 7,592,008 Membrane scaffold proteins
- 7,575,763 Membrane scaffold proteins and tethered membrane proteins
- 7,083,958 Membrane scaffold proteins
- 7,048,949 Membrane scaffold proteins
Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.
- 7,691,414 Membrane scaffold proteins
- 7,662,410 Membrane scaffold proteins and embedded membrane proteins
- 7,622,437 Tissue factor compositions and methods
- 7,592,008 Membrane scaffold proteins
- 7,575,763 Membrane scaffold proteins and tethered membrane proteins
- 7,083,958 Membrane scaffold proteins
- 7,048,949 Membrane scaffold proteins
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
Christopher I Cazzonelli et al.
eLife, 9 (2020-02-01)
Carotenoids are a core plastid component and yet their regulatory function during plastid biogenesis remains enigmatic. A unique carotenoid biosynthesis mutant, carotenoid chloroplast regulation 2 (ccr2), that has no prolamellar body (PLB) and normal PROTOCHLOROPHYLLIDE OXIDOREDUCTASE (POR) levels, was used
Ramona Faas et al.
Biotechnology reports (Amsterdam, Netherlands), 17, 45-48 (2018-01-31)
Membrane scaffold proteins (MSPs) are synthetic derivatives of apolipoprotein A-I, a major protein component of human high-density lipoprotein complexes. The most common among these is the variant MSP1D1, which has been in the focus of research on membrane mimetics in
Benjamin J Cole et al.
PLoS biology, 15(9), e2002860-e2002860 (2017-09-25)
Diverse soil-resident bacteria can contribute to plant growth and health, but the molecular mechanisms enabling them to effectively colonize their plant hosts remain poorly understood. We used randomly barcoded transposon mutagenesis sequencing (RB-TnSeq) in Pseudomonas simiae, a model root-colonizing bacterium
Yan Bao et al.
Autophagy, 14(9), 1562-1573 (2018-06-27)
Macroautophagy/autophagy is a conserved process in eukaryotes that contributes to cell survival in response to stress. Previously, we found that endoplasmic reticulum (ER) stress induces autophagy in plants via a pathway dependent upon AT5G24360/IRE1B (INOSITOL REQUIRING 1-1), an ER membrane-anchored
Ping Wang et al.
Autophagy, 16(1), 123-139 (2019-03-27)
Autophagy is a conserved catabolic process in eukaryotes that contributes to cell survival in response to multiple stresses and is important for organism fitness. In Arabidopsis thaliana, the core machinery of autophagy is well defined, but its transcriptional regulation is
Protokolle
Nanodisc technology aids membrane protein solubilization, overcoming associated challenges in diverse protein classes.
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.