MAK155

Fluorometric Sphingomyelinase Assay Kit

sufficient for 200 fluorometric tests (red fluorescence)

• Synonym(e): Neutral Sphingomyelinase Assay Kit
• UNSPSC-Code: 12161503
• NACRES: NA.84

Eigenschaften

• Verwendung: sufficient for 200 fluorometric tests (red fluorescence)
• Nachweisverfahren: fluorometric
• Relevante Krankheit(en): cancer; rheumatological diseases
• Lagertemp.: −20°C
• Angaben zum Gen: human ... SMPD2(6610) , SMPD3(55512) , SMPD4(55627) ; mouse ... SMPD2(20598) , SMPD3(58994) , SMPD4(77626) ; rat ... SMPD2(83537) , SMPD3(94338) , SMPD4(303790)

Beschreibung

Allgemeine Beschreibung

Sphingomyelinase (SMase), or sphingomyelin phosphodiesterase, catalyzes the hydrolytic cleavage of sphingolipid to phosphocholine and ceramide, a second messenger involved in multiple cellular responses including the cellular response to stress.[1] There are at least five types of SMases identified, which are classified according to their cation dependence, cellular location, and pH optima for activity, and include the acidic and neutral sphingomyelinase. Neutral SMases, along with the acidic SMases, are thought to be major contributors to the production of ceramide in response to cellular stress.[2][2] Mutations in is associated with metabolic disorders like Niemann-Pick disease B.[3]

Eignung

This kit is suitable for the detection of sphingomyelin in a variety of samples such as blood and cell extracts.

Prinzip

The Sphingomyelinase Assay Kit provides a simple and direct procedure for measuring neutral SMase activity in a variety of samples such as blood or cell extracts, or for screening SMase activators and inhibitors. SMase activity is determined by a coupled enzyme assay, which results in a fluorescent product (λex =540/λem = 590 nm) proportional to the SMase activity present.

Sicherheitshinweise

• Piktogramme: GHS08,GHS05,GHS07,GHS09
• Signalwort: Danger
• H-Sätze: H302,H317,H318,H334,H410
• P-Sätze: P261 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1
• Lagerklassenschlüssel: 10 - Combustible liquids