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Merck

MAK095

Sigma-Aldrich

Aspartate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 100 colorimetric or fluorometric tests

Nachweisverfahren

colorimetric
fluorometric

Relevante Krankheit(en)

endocrinological disorders, diabetes; neurological disorders

Lagertemp.

−20°C

Verwandte Kategorien

Allgemeine Beschreibung

Aspartate, the carboxylate anion of aspartic acid, is an acidic, non-essential amino acid involved in protein synthesis and multiple other cellular biochemical pathways. Aspartate contributes to nucleotide synthesis via the synthesis of the precursor inosine mono-phosphate. In the urea cycle, aspartate is a key metabolite, donating a nitrogen group towards the formation of urea. Aspartate is also critical for oxidative phosphorylation as part of the aspartate-malate shuttle, which transfers reducing equivalents across the mitochondrial membrane.

Anwendung

Aspartate Assay Kit has been used to measure the aspartate levels in nerve cells and Lactococcus lactis strain cultured in lysogeny broth (LB) medium.

Eignung

The Aspartate Assay Kit is suitable for aspartate detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

Prinzip

Aspartate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the aspartate present. Typical detection ranges for this kit are 2-10 nmole (colorimetric) and 0.2-1 nmole (fluorometric).

Ersetzt durch

Produkt-Nr.
Beschreibung
Preisangaben

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

188.6 °F - closed cup

Flammpunkt (°C)

87 °C - closed cup


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