Direkt zum Inhalt
Merck
Alle Fotos(2)

Wichtige Dokumente

Gesamtdokumentation anzeigen

MAK017

Sigma-Aldrich

Lactose Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(2)

About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 100 colorimetric or fluorometric tests

Anwendung(en)

cosmetics
food and beverages

Nachweisverfahren

colorimetric
fluorometric

Relevante Krankheit(en)

cancer; neonatal diseases; pediatric diseases

Lagertemp.

−20°C

Allgemeine Beschreibung

Lactose is a dissacharide formed by the condensation of one galactose and one glucose molecule. Lactose is the major sugar in the milk of most species, typically present between 2-8%. The enzyme lactase hydrolyzes lactose to its constituent monosaccharides. In neonates, glucose released via the action of lactase is a major energy source.

Anwendung

Lactose Assay Kit has been used to measure lactose content in milk.[1]

Eignung

Suitable for the direct measurement of lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.)

Prinzip

In this assay, lactose concentration is determined by an enzymatic assay, in which lactose is converted to galactose and glucose. The galactose is subsequently oxidized, resulting in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the lactose initially present. This kit is suitable for use with various biological samples and has a linear range of detection between 0.2-1.0 nmole lactose for the fluorometric assay and 2-10 nmoles lactose for the colorimetric assay.

Ersetzt durch

Produkt-Nr.
Beschreibung
Preisangaben

Ähnliches Produkt

Produkt-Nr.
Beschreibung
Preisangaben

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H317,H334

Gefahreneinstufungen

Resp. Sens. 1 - Skin Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

Flammpunkt (°F)

188.6 °F - closed cup

Flammpunkt (°C)

87 °C - closed cup

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
8L29K06240
8E26K06240
7M20K06240
7L04K06240
7J20K06240

Die passende Version wird nicht angezeigt?

Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

Suche nach COA

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Effect of high-fat diet on secreted milk transcriptome in midlactation mice.
Chen Y, et al.
Physiological Genomics, 49(12), 747-762 (2017)
Holly DeRosa et al.
eNeuro, 9(4) (2022-08-23)
Breastfeeding confers robust benefits to offspring development in terms of growth, immunity, and neurophysiology. Similarly, improving environmental complexity, i.e., environmental enrichment (EE), contributes developmental advantages to both humans and laboratory animal models. However, the impact of environmental context on maternal
Adrienne A Cheng et al.
American journal of physiology. Endocrinology and metabolism, 320(3), E438-E452 (2021-01-12)
Obesity is a prevailing problem across the globe. Women who are obese have difficulty initiating and sustaining lactation. However, the impact of genetics and diet on breastfeeding outcomes is understudied. Here we explore the effect of diet and genotype on
Yulie E Meneses et al.
Journal of dairy science, 99(5), 3396-3407 (2016-03-01)
Water scarcity is threatening food security and business growth in the United States. In the dairy sector, most of the water is used in cleaning applications; therefore, any attempt to support water conservation in these processes will have a considerable
Y Chen et al.
Physiological genomics, 49(12), 747-762 (2017-11-03)
High-fat diet (HFD) during lactation alters milk composition and is associated with development of metabolic diseases in the offspring. We hypothesized that HFD affects milk microRNA (miRNA) and mRNA content, which potentially impact offspring development. Our objective was to determine

Questions

  1. what is the substrate in the kit ? Is it lactose? can I have a look at the manual before ordering it ?

    1 answer

    1. The mechanism employed in this kit is the enzymatic conversion of the disaccharide lactose to the component monosaccharides glucose and galactose. The galactose component is then oxidized, resulting in a colorimetric/fluorimetric response.

      Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/324/674/mak017bul.pdf

      Helpful?

Reviews

No rating value

Active Filters

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.