Direkt zum Inhalt
Merck
Alle Fotos(3)

Dokumente

MAK001

Sigma-Aldrich

Alanine Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(3)

About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 100 colorimetric or fluorometric tests

Nachweisverfahren

colorimetric
fluorometric

Lagertemp.

−20°C

Allgemeine Beschreibung

Alanine is a nonessential amino acid that plays a key role in the glucose-alanine cycle between muscle tissue and the liver. In amino acid-degrading tissues such as muscle, amino groups are pooled as glutamate by transamination reactions. The amino group of glutamate is transferred to pyruvate via alanine aminotransferase, forming alanine and α-ketoglutarate. The alanine is passed into the blood and transported to the liver. This reaction is reversed in the liver where pyruvate can be used in gluconeogenesis to form glucose, which may return to other tissues through the circulatory system. Increased alanine levels correlate with higher blood pressure, energy intake, cholesterol levels, and body mass index.

Anwendung

Alanine Assay Kit has been used to determine the intracellular alanine pool.

Eignung

Suitable for alanine detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

Prinzip

In this kit, alanine concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the alanine present. Typical detection ranges for this kit are 2-10 nmole (colorimetric) and 0.2-1 nmole (fluorometric).

Ersetzt durch

Produkt-Nr.
Beschreibung
Preisangaben

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H317,H334

Gefahreneinstufungen

Resp. Sens. 1 - Skin Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

Flammpunkt (°F)

188.6 °F - closed cup

Flammpunkt (°C)

87 °C - closed cup

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

GABA transaminases from Saccharomyces cerevisiae and Arabidopsis thaliana complement function in cytosol and mitochondria.
Cao J, et al.
Yeast, 30(7), 279-289 (2013)
Mauro D P.
Amino Acids and Proteins for the Athlete: The Anabolic Edge (1997)
The complex role of branched chain amino acids in diabetes and cancer.
O'Connell T M.
Metabolites, 3(4), 931-945 (2013)
Dmitry Frank et al.
Neuropharmacology, 155, 173-184 (2019-06-04)
Post-stroke depression (PSD) is a common and serious complication following stroke. Both stroke and depression have independently been associated with pathologically elevated glutamate levels in the brain's extra-cerebral fluid (ECF). Here we evaluate an alternative therapeutic approach to PSD with
Berg J M, et al.
Biochemistry (5th Edition) (2002)
Alle zugehörigen Dokumente anzeigen

Artikel

Glutamine Metabolism in Cancer Cells

Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.

Protokolle

Enzymatic Method for Determining Alanine (Alanine Assay)

Assay protocol for the colorimetric/fluorometric detection of Alanine in biological samples using the alanine assay kit.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.