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Merck

LH20100

Supelco

Sephadex® LH-20 Size Exclusion Resin

140-550 mesh

Synonym(e):

Lipophiles Sephadex®

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise

Größe auswählen

50 G
CHF 530.00
100 G
CHF 940.00
6 X 100 G
CHF 4’040.00

CHF 530.00


Voraussichtliches Versanddatum29. März 2025


Bulk-Bestellung anfordern

Größe auswählen

Ansicht ändern
50 G
CHF 530.00
100 G
CHF 940.00
6 X 100 G
CHF 4’040.00

About This Item

UNSPSC-Code:
13111000
NACRES:
NB.21

CHF 530.00


Voraussichtliches Versanddatum29. März 2025


Bulk-Bestellung anfordern

Produktbezeichnung

Sephadex® LH-20,

Form

slurry

Qualitätsniveau

Methode(n)

LPLC: suitable

Matrix

Agarose

Aktive Matrixgruppe

polymer

Quellen

1 g swells to 4 mL (water, methanol)

Perlengröße

25-100 μm

pH Betriebsbereich

2-13

Trenntechnik

size exclusion (SEC)

Lagertemp.

room temp

Anwendung

Sephadex® LH-20 ist ein quervernetztes, auf Dextrinen basiertes Harz zur Verwendung mit organischen Lösungsmitteln als mobile Phase für die Gelpermeation, Normalphasen-Verteilungs- und Adsorptionschromatographie von Lipiden, Steroiden, Fettsäuren, Hormonen, Vitaminen usw. Des Weiteren wird es in der Gelfiltrationschromatographie, Proteinchromatographie, in Gelfiltrationsmedien, Harzen sowie Abscheidungsmedien verwendet.
Sephadex® LH-20 wird zur Isolierung des bioaktiven Polyphenolgehalts in Brombeer-Genotypen[1] und von Tryptophan aus dem wässrigen Extrakt von Lotus-Rhizomen verwendet.[2]

Rechtliche Hinweise

Sephadex is a registered trademark of Cytiva

Ersetzt durch

Produkt-Nr.
Beschreibung
Preisangaben

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

Die passende Version wird nicht angezeigt?

Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

S K Kundu et al.
Journal of lipid research, 20(7), 825-833 (1979-09-01)
Aminopropyl silica gel was prepared from porous silica gel and was used as a solid support for immunoadsorbent in the purification of anti-glycolipid antibodies. For neutral glycosphingolipids, a carboxyl function was generated by oxidation of the olefinic double bond of
Yizhong Cai et al.
Journal of chromatographic science, 43(9), 454-460 (2005-10-11)
HPLC characterization of reversed-phase (RP) high-performance liquid chromatography (HPLC) has been widely used in separation and identification of plant pigments. An effective RP-HPLC-based method is established to systematically isolate, identify, and quantitate the betalain pigments in the plants of 37
Suman B Sharma et al.
Journal of medicinal food, 14(4), 353-359 (2011-03-05)
Diabetes is accompanied by lipid abnormalities, which contribute significantly to cardiovascular morbidity and mortality in diabetic patients. We previously demonstrated the potent antihyperglycemic activity of the active principle (fraction II from Sephadex LH 20 chromatography [LH II]) isolated from ethanolic
Min Xu et al.
Biology of reproduction, 81(3), 587-594 (2009-05-29)
In vitro ovarian follicle cultures may provide fertility-preserving options to women facing premature infertility due to cancer therapies. An encapsulated three-dimensional (3-D) culture system utilizing biomaterials to maintain cell-cell communication and support follicle development to produce a mature oocyte has
The source, composition and flux of polycyclic aromatic hydrocarbons in sediments of the river Derwent, Derbyshire, U.K.
Evans, K.M., et al.
Water, Air, Soil Pollut., 51(1-2), 1-12 (1990)

Protokolle

Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.

Questions

1–6 of 6 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. What type of water can be used with Sephadex LH-20? Is it should be double deionized water or deionized water or distilled water?

    1 answer
    1. The manufacturer does not designate distilled or deionized water. However, a brief search of the literature reveals most publications note distilled water.

      Helpful?

  4. What solvents can be used with Sephadex LH-20, Product LH20100?

    1 answer
    1. Sephadex LH-20 swells in water, polar organic solvents, aqueous solvent mixtures, and mixtures of polar organic solvents.  In solvent mixtures containing both polar and non-polar organic solvents the gel takes up predominantly the polar component.   Following is a table of solvents and the approximate swelling volume.  Solvents giving a bed volume of less than 2.5 ml/g dry gel are generally not used.Solvent                           Approx. bed volume (ml/g dry gel)Dimethyl sulfoxide           4.4 - 4.6Pyridine                          4.2 - 4.4Water                             4.0 - 4.4Dimethyl formamide        4.0 - 4.4Methanol                        3.9 - 4.3Chloroform                     3.8 - 4.1Ethanol                           3.6 - 3.9Methylene dichloride       3.6 - 3.9Tetrahydrofuran              3.3 - 3.6Acetone                          2.4 - 2.6Acetonitrile                     2.2 - 2.4Benzene                         1.6 - 2.0Ethyl acetate                   1.6 - 1.8Toluene                          1.5 - 1.6

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  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. What is Sephadex LH-20, Product LH20100?

    1 answer
    1. Sephadex LH-20 is a beaded, cross-linked dextran which has been hydroxypropylated to yield a chromatographic media with both hydrophilic and lipophilic character.  Due to its dual character, LH-20 swell in water and a number of organic solvents.  The particle size and exclusion limit vary depending on the solvent used for swelling.  The swollen resin can be used for gel filtration separations of both hydrophilic and lipophilic compounds.

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