L2023

Leupeptin trifluoroacetate salt

powder, ≥90% (HPLC)

• Synonym(e): Acetyl-Leu-Leu-Arg-al -trifluoracetat (Salz)
• Empirische Formel (Hill-System): C₂₀H₃₈N₆O₄ · xC₂HF₃O₂
• CAS-Nummer: 147385-61-3
• Molekulargewicht: 426.55 (free base basis)
• MDL-Nummer: MFCD00133458
• UNSPSC-Code: 12352202
• PubChem Substanz-ID: 329817397
• NACRES: NA.77

Eigenschaften

• Produktbezeichnung: Leupeptin trifluoroacetate salt, ≥90% (HPLC), microbial
• Biologische Quelle: microbial
• Qualitätsniveau: 200
• Assay: ≥90% (HPLC)
• Form: powder
• Löslichkeit: H₂O: 10 mM (Solutions are stable only a few hours. Stock solutions are stable up to 6 months at −20 °C.)
• Lagertemp.: −20°C
• SMILES String: OC(=O)C(F)(F)F.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O
• InChI: 1S/C20H38N6O4.C2HF3O2/c1-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22;3-2(4,5)1(6)7/h11-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23);(H,6,7)/t15-,16-,17-;/m0./s1
• InChIKey: WUHGBZVQELGOMH-FRKSIBALSA-N

Beschreibung

Biochem./physiol. Wirkung

Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM. There have been numerous studies using leupeptin to protect against hearing loss caused by acoustic overstimulation or the ototoxic antibiotic gentamicin. (Loss of cochlear hair cells is believed to be mediated by calpain.)

Hinweis zur Analyse

Leupeptin gives multiple peaks on HPLC because of the formation of tautomeric isomers in solution. Majority of contaminating peptide is racemized leupeptin.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)