Direkt zum Inhalt
Merck
Alle Fotos(1)

Wichtige Dokumente

L1668

Sigma-Aldrich

Lektin aus Phaseolus vulgaris (rote Kidneybohne)

Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

Synonym(e):

Phaseolus vulgaris Agglutinin, PHA

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise

Größe auswählen

5 MG
CHF 75.80

CHF 75.80

Voraussichtliches Versanddatum09. April 2025

Alle Fotos(1)

Größe auswählen

Ansicht ändern
5 MG
CHF 75.80

About This Item

UNSPSC-Code:
12352202
NACRES:
NA.32

CHF 75.80

Voraussichtliches Versanddatum09. April 2025

Qualitätsniveau

200

Produktlinie

BioReagent

Form

lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

Wirksamkeit

≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

Methode(n)

cell culture | mammalian: suitable

Farbe

white to off-white

Löslichkeit

PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)

Lagertemp.

2-8°C

Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich

Verwandte Kategorien

Anwendung

Lektin aus Phaseolus vulgaris (Gartenbohnen) hat folgende Anwendungen:
  • Stimulation von Differenzierungscluster (CD4+)-T-Zellen[1]
  • Aktivierung von primären Lymphozyten des peripheren Bluts[2]
  • Stimulation von mononuklearen Zellen des peripheren Bluts[3][4]

Biochem./physiol. Wirkung

PHA besteht aus zwei Molekularspezies, einem Erythroagglutinin (PHA-E) mit niedriger mitogener und hoher erythroagglutinierender Aktivität sowie einem Leucoagglutinin (PHA-L) mit hoher mitogener und hoher leucoagglutinierender Aktivität, aber sehr niedriger erythroagglutinierender Aktivität.
PHA-E ist nicht blutgruppenspezifisch, die Agglutination kann aber durch bestimmte Oligosaccharide gehemmt werden. PHA-P ist die Proteinform von PHA vor Trennung und Aufreinigung von Erythroagglutinin und Leucoagglutinin. PHA-M ist die Mucoproteinform. Konjugate werden aus den entsprechenden aufgereinigten Lektinen zubereitet.

Verpackung

Vertrieb auf Basis des Lektingehalts

Hinweis zur Analyse

Stärke und Löslichkeit ausgedrückt in mg Lektin/ml

Ähnliches Produkt

Produkt-Nr.
Beschreibung
Preisangaben

08168

Timestrip Plus™ 8 °C

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000408863
0000406766
0000384022
0000354443
SLCM5481

Die passende Version wird nicht angezeigt?

Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

Suche nach COA

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Cellular RelB interacts with the transactivator Tat and enhance HIV-1 expression
Wang M, et al.
Retrovirology, 15(1), 65-65 (2018)
Ema T Crooks et al.
PLoS pathogens, 14(5), e1007024-e1007024 (2018-05-03)
The extensive glycosylation of HIV-1 envelope (Env) glycoprotein leaves few glycan-free holes large enough to admit broadly neutralizing antibodies (bnAb). Consequently, most bnAbs must inevitably make some glycan contacts and avoid clashes with others. To investigate how Env glycan maturation
Nina Thakkar et al.
Antimicrobial agents and chemotherapy, 53(2), 631-638 (2008-12-03)
The present studies were conducted to better define the mechanism of action of polyethylene hexamethylene biguanide (PEHMB) (designated herein as NB325), which was shown in previous studies to inhibit infection by the human immunodeficiency virus type 1 (HIV-1). Fluorescence-activated flow
Frank W King et al.
Stem cells and development, 20(3), 475-484 (2010-07-14)
Pluripotent human embryonic stem cells (hESCs) provide an unprecedented opportunity for the study of human tissue development, and the development of cell-based therapies for human disease. To realize these potential advances, however, methods for monitoring expression of intracellular proteins in
Cody J Warren et al.
PLoS biology, 17(6), e3000304-e3000304 (2019-06-11)
Individuals chronically infected with HIV-1 harbor complex viral populations within their bloodstreams. Recently, it has come to light that when these people infect others, the new infection is typically established by only one or a small number of virions from
Alle zugehörigen Dokumente anzeigen

Artikel

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Questions

1–7 of 7 Questions  

  1. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 answer

    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

      Helpful?

  2. How should the PHA solution using Phytohemagglutinin PHA-P powder be prepared for cell culture?

    1 answer

    1. The stock solution of this product can be prepared in phosphate buffered saline at pH 6.8, resulting in a clear to slightly hazy solution at a concentration of 5 mg per mL. This stock solution can then be diluted in cell culture medium to a working concentration of 10 micrograms of lectin per mL for use in peripheral blood cell culture.

      Helpful?

  3. What is the shelf life once reconstituted in PBS?

    1 answer

    1. After reconstitution in PBS, this product can be stored at 2-8°C for 2-4 weeks. PHA loses approximately 30% activity after 1 month. Stock solutions of at least 1 mg/mL can be stored in frozen aliquots. Do not dilute to the working concentration until ready to use. Vortex stock solutions prior to use.

      Helpful?

  4. What is the mitogenic activity of Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder?

    1 answer

    1. The minimum specification for mitogenic activity of this product is  < or = 10 micrograms lectin/mL of medium. The reported specification is PASS. A specific value is not given.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. How can Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder, be dissolved?

    1 answer

    1. Product No. L1668 is solubility tested using 5 mg per mL of phosphate buffered saline (PBS), pH 6.8; this yields a clear to slightly hazy, colorless to light yellow solution.

      Helpful?

  7. What is the solution stability for Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder?

    1 answer

    1. The solution stability was not evaluated by Sigma-Aldrich; however, references indicate media containing dilute PHA can be stored refrigerated for 2-4 weeks. PHA loses approximately 30% activity after 1 month. Stock solutions can be stored in frozen aliquots.

      Helpful?

Reviews

No rating value

Active Filters

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.