I0523

Interleukin-2 aus mouse

IL-2, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(e): Human Interleukin-2, rekombinant
• MDL-Nummer: MFCD00164816
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: ≤0.2 ng/mL ED₅₀
• Qualität: endotoxin tested
• Mol-Gew.: 17.2 kDa
• Verpackung: pkg of 5X20 μg; pkg of 20 μg
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: ≤1.000 EU/μg
• Farbe: white
• UniProt-Hinterlegungsnummer: P04351
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... Il2(16183)

Beschreibung

Allgemeine Beschreibung

IL-2 (interleukin-2) codes for a pro-inflammatory cytokine. IL-2 is mostly expressed in T helper cells.

Anwendung

Interleukin-2 from mouse has been used to induce sepsis in rat model. It has also been used in dendritic epidermal T cell culture.

Biochem./physiol. Wirkung

Interleukin-2 (IL-2) is an immunomodulatory factor produced by certain subsets of T lymphocytes. This lymphokine promotes long-term growth of activated T cells and related cell types. IL-2 plays a role in the activation and proliferation of natural killer cells, induces γ-interferon and B cell growth factor secretion, and modulates the expression of the IL-2 receptor. IL-2 expression is known to reduce with age and results in influenza infection, neoplasms and lethal autoimmunity. IL-2 participates in T cell proliferation and differentiation and controls the homeostasis of T cell. As a part of immune response, IL-2 stimulates the action of T helper cell 1 and 2 along with endothelial white blood cells of skeletal muscles. IL-2 regulation is controlled by c-rel protein. IL-2 is a neurotrophic molecule and has special functions in cortical, hippocampal, septal, cerebellar and retinal neurons.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in 10 mM sodium citrate, pH 4.0.

Hinweis zur Analyse

Activity was determined by dose-dependent stimulation of murine CTLL-2 cells. ED₅₀ : ≤ 0.2 ng/ml (corresponds to a specific activity of 5 × 10⁶ units/mg)

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)