I5271

IL-1β mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(e): IL-1β, Interleukin-1β, Lymphocyte activating factor
• MDL-Nummer: MFCD00164815
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Produktbezeichnung: Interleukin-1β from mouse, IL-1β, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: ≤0.010 ng/mL ED₅₀
• Qualität: endotoxin tested
• Mol-Gew.: 17.5 kDa (containing 153 amino acid residues)
• Verpackung: pkg of 5X5 μg; pkg of 5 μg
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: ≤0.1ng/mg
• UniProt-Hinterlegungsnummer: P10749
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... Il1b(16176)

Beschreibung

Allgemeine Beschreibung

Interleukin-1β (IL-1β) cytokine is generated by monocytes, dendritic cells, macrophages and keratinocytes. Pro-IL-1β, an IL-1β precursor undergoes proteolytic cleavage to produce its active form of 17 kDa.[1]

Anwendung

Interleukin-1β from mouse has been used:

• as a proinflammatory agent,[2] to induce inflammatory signaling cascades mediated cellular damage[3]
• to study its effect on fetuin A-induced inflammatory response in podocyte[4]
• to check its effect on the expression of mitogen-activated protein kinase 4 using 3T3-L1 adipocytes[5]

Biochem./physiol. Wirkung

Interleukin-1β (IL-1β) is a potent inflammatory cytokine,[1] and mediates inflammation through proinflammatory molecules: cyclooxygenase-2 (COX2) and nitric oxide.[6] IL-1β release is stimulated by injury and increased levels are observed in response to severe pain.[7] It has its main function in T-cell activation, antigen recognition and T-helper cell 17 cell development. IL-1β is also responsible for the formation of IL-17 and IL-22 by nature killer T (NKT) cells and NK cells. It is known to induce inflammation in psoriasis and rheumatoid arthritis.[1] IL-1β controls intercellular adhesion molecule-1 expression. It is significantly associated with bone damage and vascular injury.[8]

Physikalische Form

Lyophilized from 0.2 μm filtered solution in 10mM Sodium Citrate, pH 4.0

Hinweis zur Analyse

The biological activity is measured by the dose-dependent stimulation of murine D10G4.1 cells.

Sonstige Hinweise

The two closely related agents, Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) bind to the same cell surface receptor, elicit nearly identical biological responses and yet share 25% homology in their amino acid sequence.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable