GE17-0921-07
IMAC Sepharose™ 6 Fast Flow
Cytiva 17-0921-07, pack of 25 mL
About This Item
Empfohlene Produkte
Verpackung
pack of 25 mL
Hersteller/Markenname
Cytiva 17-0921-07
Matrix
6% cross-linked agarose
Partikelgröße
45-165 μm
Durchschnittlicher Durchmesser
90 μm
cleaning in place
2-14(Medium uncharged with metal ions.)
Arbeitsbereich
3-12
Kapazität
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
Eignung
suitable for bioprocess medium
Allgemeine Beschreibung
Anwendung
IMAC Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC Sepharose™ 6 Fast FLow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target Protein.
The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.
IMAC Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, IMAC Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support
Leistungsmerkmale und Vorteile
- Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
- Convenient purification of histidine-tagged Proteins, also when Ni2+ is not the best choice of metal ion
- As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- Available in the convenient and time-saving prepacked HiTrap® and HiPrep™ formats.
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
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Artikel
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
How to optimize purification of histidine-tagged proteins using Cytiva products.
Optimierung der Aufreinigung von Histidin-markierten Proteinen mit Cytiva-Produkten.
Protokolle
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.
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