E1388

β-Endothelial Cell Growth Factor human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(e): ECGS, β-ECGF
• CAS-Nummer: 105843-51-4
• MDL-Nummer: MFCD00131012
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Produktbezeichnung: β-Endothelial Cell Growth Factor human, β-ECGF, recombinant, expressed in E. coli, suitable for cell culture
• Biologische Quelle: human
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: 0.03-0.3 ng/mL ED₅₀/EC₅₀
• Qualität: endotoxin tested
• Mol-Gew.: predicted mol wt ~17 kDa
• Verpackung: pkg of 25 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: ≤1.0 EU/μg protein Endotoxin level (LAL test)
• UniProt-Hinterlegungsnummer: P49765
• Lagertemp.: −20°C
• Angaben zum Gen: human ... VEGFB(7423)

Beschreibung

Allgemeine Beschreibung

The β-endothelial cell growth factor (b-ECGF) gene encodes an angiogenic factor,[1] and is mapped to human chromosome 11q13.1.[2] Recombinant, human b-endothelial cell growth factor (b-ECGF) is a 14 amino acid N-terminal extension of acidic fibroblast growth factor. It is expressed in Escherichia coli. The 154 amino acid recombinant protein, generated after removal of the initiation methionine, has a predicted molecular mass of about 17 kDa.

Anwendung

β-Endothelial Cell Growth Factor human has been used:

• in cell death assay[3]
• in endothelial cell culture[4]
• in human umbilical vascular endothelial cell (HUVEC) culture[5]

Biochem./physiol. Wirkung

β-Endothelial cell growth factor (β-ECGF) is a potent endothelial cell mitogen and its mitogenic activity is potentiated by heparin. β-ECGF stimulates the growth of quiescent HUVEC cells in culture. β-ECGF is associated with lipid and glucose metabolism.[2] This growth factor induces neovascularization in disease conditions. Upregulation of β-ECGF is observed in diabetic retinopathy,[6] and polycystic ovary syndrome.[7] It is also associated with tumor proliferation and invasion. β-ECGF increases fatty acid transport proteins expression to improve endothelial mediated lipid transpor.[6]

Sonstige Hinweise

14 amino acid, N-terminal extended form of human FGF-acidic.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in 10 mM MOPS, 50 mM ammonium sulfate, 0.2 mM DTT, and 0.2 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine.

Hinweis zur Analyse

The proliferative activity is tested in culture by using quiescent NR6R-3T3 fibroblasts.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)