FTC-238

94060902, human thyroid (lung metastasis), Polymorphic

• UNSPSC-Code: 41106514

Eigenschaften

• product name: FTC-238, 94060902
• Biologische Quelle: human thyroid (lung metastasis)
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Polymorphic
• Produkte: Expression of 5′ - Deiodinase Type I
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): metastasis
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human follicular thyroid carcinoma, lung metastasis

Beschreibung der Zelllinie

FTC-238 was established from a lung metastasis of a follicular thyroid carcinoma from a 42-year-old male. The cells are polymorphic showing flat polygonal to spindle-like morphologies. Complex chromosomal changes were detected as well as a p53 mutation. FTC-133 (Sigma Catalogue number 94060901), FTC-236 (Sigma Catalogue number 06030202) and FTC-238 (Sigma Catalogue number 94060902) were all derived from the same individual, a 42 year old male with follicular thyroid cancer. This has been confirmed by short tandem repeat STR-PCR analysis at ECACC The Y chromosome could not be detected in these cell line by (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell lines are identical to the source provided by the depositor based on the STR-PCR analysis.

Anwendung

FTC-238 has been used to study the function of human thyroid and development of thyroid cancer.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 9,10
THO1: 9.3
TPOX: 9
vWA: 15,18

Nährmedium

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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