MG-63 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86051601, human bone, Fibroblast

• Synonym(e): MG63 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human bone
• Beschreibung: Human osteosarcoma
• Verpackung: tube of 5 μg 86051601-DNA-5UG; pkg of vial of cells 86051601-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 66; hypotriploid
• Morphologie: Fibroblast
• Produkte: Inducible interferon
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human osteosarcoma

Beschreibung der Zelllinie

From an osteosarcoma of a 14 year old male. Produces high yields of interferon following superinduction with polyinosinic acid-polycytidilic acid, cyclohexamide and actinomycin. Antigenically, MG-63 interferon is more closely related to human fibroblast than to leukocyte interferon.

Anwendung

Human MG-63 cell line has been used:

• to investigate the cytotoxicity of acrylic bone cement extracts on MG-63 cells[1]
• to fabricate three-dimensional (3-D) niches and study the cell viability, adhesion and proliferation of MG-63 cells[2]
• as a positive control for immunostaining procedure[3]
• to study the interactions and effects of lysophosphatidate functionalized titanium on MG-63 cells[4]

Interferon-induction studies

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 9.3
TPOX: 8,11
vWA: 16,19

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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