C-12212

Human Dermal Microvascular Endothelial Cells (HDMEC)

Adult, 500,000 cryopreserved cells

• Synonym(e): HDMEC, Microvascular Endothelial Cells
• UNSPSC-Code: 41106514
• NACRES: NA.81

Eigenschaften

• Biologische Quelle: human dermis (adult)
• Verpackung: pkg of 500,000 cells
• Morphologie: ( endothelial)
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Allgemeine Beschreibung

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Ursprung der Zelllinie

Skin

Anwendung

Primary Human Dermal Microvascular Endothelial Cells (HDMEC) are isolated from the dermis of adult skin (different locations) and are provided in a cryopreserved format. Since the dermis contains blood and lymphatic capillaries, HDMEC comprise Blood and Lymphatic Microvascular Endothelial Cells. Both have a common origin and can be identified by several markers.

Qualität

Rigid quality control tests are performed for each lot of Microvascular Endothelial Cells. They are tested for cell morphology and cell type specific markers, e.g. von Willebrand Factor (vWF), CD31 and Podoplanin using flow cytometric analyses. Growth performance is tested through multiple passages up to 15 population doublings (PD) without antibiotics or antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warnhinweis

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subkultur-Routine

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Sonstige Hinweise

Recommended Plating Density: 10000 - 20000 cells per cm²Passage After Thawing: P2Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Empfohlene Produkte

Recommended Primary Cell Culture Media:Link

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable