C-12262

Human Dermal Microvascular Endothelial Cells (HDMEC)

Adult, 500,000 proliferating cells

• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human dermis
• Morphology: ( endothelial)

Eigenschaften

• biological source: human dermis
• packaging: pkg of 500,000 cells
• morphology: ( endothelial)
• technique(s): cell culture | mammalian: suitable
• shipped in: ambient
• storage temp.: room temp

Beschreibung

General description

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Application

Primary Human Dermal Microvascular Endothelial Cells (HDMEC) are isolated from the dermis of adult skin (different locations) and are provided in a proliferating format. Since the dermis contains blood and lymphatic capillaries, HDMEC comprise Blood and Lymphatic Microvascular Endothelial Cells. Both have a common origin and can be identified by several markers.

Biochem/physiol Actions

Skin

Preparation Note

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Analysis Note

Rigid quality control tests are performed for each lot of Microvascular Endothelial Cells. They are tested for cell morphology and cell type specific markers, e.g. von Willebrand Factor (vWF), CD31 and Podoplanin using flow cytometric analyses. Growth performance is tested through multiple passages up to 15 population doublings (PD) without antibiotics or antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 10000 - 20000 cells per cm²Passage After Thawing: P2Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Recommended Primary Cell Culture Media:Link

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 3
• Flammpunkt (°F): No data available
• Flammpunkt (°C): No data available