This product is a conjugate of 5'-ATP to crosslinked 4% beaded agarose (activated by cyanogen bromide), via the C-8 atom of 5'-ATP. A 9-atom matrix spacer arm connects the ATP and the agarose. Please see the link below to review the product datasheet for more information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/174/887/a2767pis.pdf
Alle Fotos(3)
Wichtige Dokumente
A2767
Adenosin 5′-triphosphat-Agarose
lyophilized powder
Synonym(e):
5′-ATP agarose
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Größe auswählen
1 ML
CHF 151.00
5 ML
CHF 580.00
25 ML
CHF 2’460.00
CHF 151.00
Voraussichtliches Versanddatum01. Juni 2025
Alle Fotos(3)
Größe auswählen
Ansicht ändern
1 ML
CHF 151.00
5 ML
CHF 580.00
25 ML
CHF 2’460.00
About This Item
CHF 151.00
Voraussichtliches Versanddatum01. Juni 2025
Empfohlene Produkte
Biologische Quelle
plant (Sea weed)
Form
lyophilized powder
Kennzeichnungsgrad
1-5 μmol per mL
Matrix
cross-linked 4% beaded agarose
Matrixaktivierung
cyanogen bromide
Matrixanbindung
C-8
Matrix-Spacer
9 atoms
Lagertemp.
−20°C
SMILES String
[X]Nc1ncnc2[n](cnc21)[C@@H]3O[C@@H]([C@H]([C@H]3O)O)CO[P](=O)(O[P](=O)(O[P](=O)(O)O)O)O
Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich
Verwandte Kategorien
Allgemeine Beschreibung
Adenosin-5′-triphosphat–Agarose (5′-ATP-Agarose) ist ein Konjugat von 5′-ATP an vernetzte 4%ige kugelförmige Agarose (aktiviert durch Cyanogenbromid) über das C-8-Atom von 5′-ATP. 5′-ATP-Agarose kann in der Affinitätsaufreinigung von verschiedenen Proteinen und Enzymen wie CDK2 (cyclin-dependent kinase 2), Hitzeschockproteine und Kryptochromen angewandt werden.
Anwendung
Adenosin-5′-triphosphat-Agarose (5′-ATP-Agarose) wird in der Affinitätschromatographie zur Aufreinigung der Uridinkinase aus Ehrlich-Aszites-Tumorzellen verwendet.
Adenosin-5′-triphosphat–Agarose (5′-ATP-Agarose) kann in der Affinitätsaufreinigung von verschiedenen Proteinen und Enzymen wie CDK2 (cyclin-dependent kinase 2), Hitzeschockprotein-70 (Hsp-70) und Kryptochromen angewandt werden. 5′-ATP-Agarose wird in der Affinitätschromatografie zur Aufreinigung der Uridinkinase aus Ehrlich-Aszites-Tumorzellen verwendet.
Physikalische Form
Lyophilisiertes Pulver stabilisiert mit Lactose
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
Hier finden Sie alle aktuellen Versionen:
Analysenzertifikate (COA)
Lot/Batch Number
Die passende Version wird nicht angezeigt?
Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
C Prodromou et al.
The EMBO journal, 18(3), 754-762 (1999-02-02)
The in vivo function of the heat shock protein 90 (Hsp90) molecular chaperone is dependent on the binding and hydrolysis of ATP, and on interactions with a variety of co-chaperones containing tetratricopeptide repeat (TPR) domains. We have now analysed the
M Brungs et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(1), 107-111 (1995-01-03)
5-Lipoxygenase (5-LO; EC 1.13.11.34) activity in the human monocytic cell line Mono Mac 6 was upregulated by combined treatment with transforming growth factor beta 1 (TGF-beta) and 1,25-dihydroxyvitamin D3 (VD3). In undifferentiated cells, 5-LO enzyme activity was undetectable. After the
Christian Feisst et al.
The Journal of pharmacology and experimental therapeutics, 315(1), 389-396 (2005-07-15)
Myrtucommulone (MC) and semimyrtucommulone (S-MC) are unique oligomeric, nonprenylated acylphloroglucinols contained in the leaves of myrtle (Myrtus communis). Although extracts of myrtle have been traditionally used in folk medicine for the treatment of various disorders, studies addressing select cellular or
Erin E Nicklow et al.
The Journal of biological chemistry, 295(2), 552-569 (2019-12-07)
Cells employ a vast network of regulatory pathways to manage intracellular levels of reactive oxygen species (ROS). An effectual means used by cells to control these regulatory systems are sulfur-based redox switches, which consist of protein cysteine or methionine residues
C Greiner et al.
British journal of pharmacology, 164(2b), 781-793 (2011-04-22)
5-Lipoxygenase (5-LO) is the key enzyme in the biosynthesis of pro-inflammatory leukotrienes (LTs) representing a potential target for pharmacological intervention with inflammation and allergic disorders. Although many LT synthesis inhibitors are effective in simple in vitro test systems, they frequently
-
Where is the linker attached on the ATP molecule
1 answer-
Helpful?
-
-
How can I hydrate Product A2767, Adenosine 5´-triphosphate-Agarose?
1 answer-
The resin can be hydrated by placing it in excess water, approximately 50 mL per g of resin, for at least 30 minutes. Remove the lactose stabilizer by washing the resin on a Buchner funnel with gentle vacuum, using approximately 100 mL of water per g of resin. Do not allow the resin to dry. Resuspend the resin in excess water or starting buffer to pack the column bed.
Helpful?
-
-
How can I regenerate Product A2767, Adenosine 5´-triphosphate-Agarose?
1 answer-
Since N6-[N-(6-aminohexyl)-carbamyl]-ADP is a substrate for acetate kinase and pyruvate kinase, these enzymes can be used to regenerate ATP from ADP on the resin under conditions favoring the reverse reaction. Wash the resin with 20 volumes of 50 mM Tris HCl buffer (pH 8.2), with 0.2 mM EDTA and 100 mM KCl. Incubate the resin overnight at 2-8°C in an ATP-regenerating mixture containing 0.2 mM phosphoenol pyruvate, pyruvate kinase (10 units per mL of resin), 5 mM MgCl2, 0.2 mM EDTA and 100 mM KCl in 50 mM Tris HCl buffer (pH 8.2). Wash the resin with 25 column volumes of 2 mM ATP in 2 M KCl and reequilibrate the resin with 25 column volumes of sample buffer. Alternatively, the ATP regenerating mixture can contain 20 mM acetyl phosphate, acetate kinase (6.8 units per mL of resin), and 3 mM MgCl2 in 100 mM Tris HCl (pH 7.6). If acetate kinase is used, prewash the resin with 25 column volumes of 100 mM Tris HCl (pH 7.6), but use the same final wash and reequilibration steps as given for pyruvate kinase.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
How can I elute my proteins from Product A2767, Adenosine 5´-triphosphate-Agarose?
1 answer-
Specifically bound proteins can be eluted with 10-100 mM ATP or ADP. Nonspecifically bound proteins can be eluted with 2 M NaCl or KCl in water or 7 M urea.
Helpful?
-
-
Can I store Product A2767, Adenosine 5´-triphosphate-Agarose, after I have hydrated it?
1 answer-
For the short term, it is possible to store the hydrated resin in 20% ethanol and dilute buffer at 2-8°C. In general, the hydrated resin can be stored refrigerated in water or buffer containing a bacteriostat, such as 0.02% sodium azide or thimerosal. Do not autoclave or freeze the hydrated resin. The resin can be used several times without loss of effectiveness. However, the ATP will slowly hydrolyze to ADP over time, and under certain conditions this process may be accelerated during usage. The resin can be regenerated; please see the regeneration FAQ for this product.
Helpful?
-
Active Filters
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.
