94742

Vanadyl-Ribonukleosid-Komplexe -Lösung

BioReagent, for molecular biology, 200 mM

• Synonym(e): Ribonucleosid-Vanadyl-Komplexe
• UNSPSC-Code: 12352200
• NACRES: NA.52

Eigenschaften

• Qualität: Molecular Biology; for molecular biology
• Produktlinie: BioReagent
• Form: liquid
• Konzentration: 200 mM
• Verunreinigungen: oxovanadium V
• Eignung: in accordance for ribonuclease inhibition test
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.[1]

Vanadyl ribonucleoside complexes solution is an inhibitor for ribonuclease (RNase).[2] Use of vanadyl ribonucleoside complexes exhibits protection from degradation by RNase in tissue, genomic, RNA and protein samples.[3]

Anwendung

Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.

Vanadyl ribonucleoside complexes solution has been used:

• as a component of polysome lysis buffer[4] and fluorescence in situ hybridization (FISH) buffer[5][6]
• as a RNase inhibitor for the isolation of endogenous YTH domain-containing 2 protein (YTHDC2) from mouse testes[7]

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable