91228

Trichloressigsäure

BioUltra, ≥99.5% (T)

• Synonym(e): TCA
• Lineare Formel: Cl₃CCOOH
• CAS-Nummer: 76-03-9
• Molekulargewicht: 163.39
• Beilstein: 970119
• EG-Nummer: 200-927-2
• MDL-Nummer: MFCD00004177
• UNSPSC-Code: 12352101
• PubChem Substanz-ID: 57653428
• NACRES: NA.25

Eigenschaften

• Qualität: biotech. grade
• Dampfdichte: <1 (vs air)
• Dampfdruck: 1 mmHg ( 51 °C)
• Produktlinie: BioUltra
• Assay: ≥99.5% (T)
• Form: crystals; flakes
• Verunreinigungen: insoluble matter, passes filter test
• Glührückstand: ≤0.05%
• Brechungsindex: n20/D 1.62 (lit.)
• pH-Wert: <1.0 (25 °C, 0.5 M in H₂O)
• bp: 196 °C (lit.)
• mp (Schmelzpunkt): 54-58 °C (lit.)
• Löslichkeit: H₂O: 0.5 M at 20 °C, clear, colorless
• Dichte: 1.62 g/mL at 25 °C (lit.)
• Anionenspuren: chloride (Cl^-): ≤30 mg/kg; nitrate (NO₃^-): ≤20 mg/kg; phosphate (PO₄^3-): ≤10 mg/kg; sulfate (SO₄^2-): ≤100 mg/kg
• Kationenspuren: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• SMILES String: OC(=O)C(Cl)(Cl)Cl
• λ: 0.5 M in H₂O
• UV-Absorption: λ: 260 nm A_max: 1.60; λ: 280 nm A_max: 0.15
• InChI: 1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)
• InChIKey: YNJBWRMUSHSURL-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Trichloroacetic acid (TCA) is derived from Trichloroethylene (TCE) metabolism.[1] It is used as an acid decalcifying agent. TCA is used as a fixative for nuclear staining and protein precipitation.[2]

Anwendung

Traditionell zur Fällung von Proteinen verwendet. Zur Bestimmung der Proteinkonzentration durch quantitative Fällung. Zum Entkalken und als Fixationsmittel in der Mikroskopie verwendet.

Trichloroacetic acid (TCA) has been used:

• to determine its effect on avian embryos during an exposure window encompassing cardiac specification[3]
• as a cell fixative[4][5][6]
• to terminate the O-acetylserine (thiol)lyase (OASTL) enzymatic assay[6]
• for the precipitation of acidic ribosomal proteins (RPs)[7]

Sonstige Hinweise

Concentration of protein by quantitative precipitationcitation; Cytochemical demonstration of DNA with a thiazine dyecitation

Concentration of protein by quantitative precipitation[8]; Cytochemical demonstration of DNA with a thiazine dye[9]

Sicherheitshinweise

• Piktogramme: GHS05,GHS09
• Signalwort: Danger
• H-Sätze: H314,H410
• P-Sätze: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Gefahreneinstufungen: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A
• Lagerklassenschlüssel: 8B - Non-combustible corrosive hazardous materials
• WGK: WGK 2
• Flammpunkt (°F): >235.4 °F - closed cup
• Flammpunkt (°C): > 113 °C - closed cup
• Persönliche Schutzausrüstung: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges