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Merck

88793

Sigma-Aldrich

Atto 532 NHS-Ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym(e):

Atto 532

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1 MG
CHF 346.00
5 MG
CHF 1’690.00

CHF 346.00


Voraussichtliches Versanddatum10. April 2025


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1 MG
CHF 346.00
5 MG
CHF 1’690.00

About This Item

MDL-Nummer:
UNSPSC-Code:
12352108
NACRES:
NA.32

CHF 346.00


Voraussichtliches Versanddatum10. April 2025


Bulk-Bestellung anfordern

Produktlinie

BioReagent

Qualitätsniveau

Assay

≥90% (HPLC)
≥90% (degree of coupling)

Form

powder

Hersteller/Markenname

ATTO-TEC GmbH

λ

in methanol: water (1:1) (with 0.1% perchloric acid)

UV-Absorption

λ: 532-538 nm Amax

Eignung

suitable for fluorescence

Lagertemp.

−20°C

Allgemeine Beschreibung

Atto 532 NHS ester is a fluorescent dye related to the well-known laser dye, Rhodamine 6G. The fluorescence activity is excited efficiently at the 515-545nm range. A suitable excitation source for Atto 532 is the 532 nm output of the frequency-doubled Nd: YAG laser.

Anwendung

Atto 532 NHS ester is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, and STED. In addition, the dye is used in flow cytometry (FACS) and fluorescence in-situ hybridization (FISH) methods.

Leistungsmerkmale und Vorteile

Characteristic features of the Atto 532 NHS ester are:
  • Strong Absorption.
  • High Fluorescence quantum yield.
  • High Photostability.
  • Excellent water solubility.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Rumelo Amor et al.
Scientific reports, 4, 7359-7359 (2014-12-09)
Standing-wave excitation of fluorescence is highly desirable in optical microscopy because it improves the axial resolution. We demonstrate here that multiplanar excitation of fluorescence by a standing wave can be produced in a single-spot laser scanning microscope by placing a
Wonchul Shin et al.
Cell, 173(4), 934-945 (2018-04-03)
Fusion is thought to open a pore to release vesicular cargoes vital for many biological processes, including exocytosis, intracellular trafficking, fertilization, and viral entry. However, fusion pores have not been observed and thus proved in live cells. Its regulatory mechanisms and
Roland Bienert et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 12(3), 510-517 (2011-02-03)
H(+)-ATP synthases are molecular machines which couple transmembrane proton transport with ATP synthesis from ADP and inorganic phosphate by a rotational mechanism. Single-pair fluorescence resonance energy transfer (spFRET) in single molecules is a powerful tool to analyse conformational changes. It
Toshiro Saito et al.
Nanotechnology, 22(44), 445708-445708 (2011-10-13)
We fabricated platinum bowtie nanostructure arrays producing fluorescence enhancement and evaluated their performance using two-photon photoluminescence and single-molecule fluorescence measurements. A comprehensive selection of suitable materials was explored by electromagnetic simulation and Pt was chosen as the plasmonic material for
Andrea Armbrüster et al.
FEBS letters, 579(9), 1961-1967 (2005-03-29)
The ability of subunit C of eukaryotic V-ATPases to bind ADP and ATP is demonstrated by photoaffinity labeling and fluorescence correlation spectroscopy (FCS). Quantitation of the photoaffinity and the FCS data indicate that the ATP-analogues bind more weakly to subunit

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