This product may be stored at room temperature.
Wichtige Dokumente
P1379
TWEEN® 20
viscous liquid
Synonym(e):
Polyethylenglykolsorbitanmonolaurat, Polyoxyethylensorbitanmonolaurat
About This Item
Empfohlene Produkte
Beschreibung
non-ionic
Qualitätsniveau
Assay
>40% (GC)
Form
viscous liquid
Mol-Gew.
~1228
Zusammensetzung
lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
Konzentration
≥40.0% (GC)
Methode(n)
DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable
Verunreinigungen
≤3.0% water
Brechungsindex
n20/D 1.468 (lit.)
CMC
0.06 mM (20-25°C)
Säurezahl
≤2.2 mg/g
Hydroxylzahl
96‑108 mg/g
Übergangstemp.
cloud point 76 °C
Dichte
1.095 g/mL at 25 °C (lit.)
HLB
16.7
Eignung
complies for IR spectroscopy
SMILES String
CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
InChI
1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
InChIKey
HMFKFHLTUCJZJO-UHFFFAOYSA-N
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Allgemeine Beschreibung
Tenside mit sowohl hydrophilen als auch hydrophoben Komponenten lassen sich in zwei Hauptkategorien unterteilen: ionische, die in Wasser ionisieren (anionisch, kationisch oder ampholytisch), und nichtionisch, die nicht ionisieren. Bei diesem Produkt handelt es sich um ein nichtionisches Tensid für verschiedene Anwendungen wie Emulgierung, Dispersion, Solubilisierung und zur Verwendung als Reinigungsmittel. Darüber hinaus kann es als Solubilisierungsmittel für Membranproteine dienen.
Anwendung
TWEEN 20 wurde als Blockierungsmittel für membranbasierte Immunassays bei einer typischen Konzentration von 0,05 % verwendet. TWEEN 20 kann zur Lyse von Säugetierzellen bei einer Konzentration von 0,005 bis 0,5 % zum Einsatz kommen.
Leistungsmerkmale und Vorteile
- Sehr vielseitig einsetzbares Tensid für Ihre Forschung in der Biowissenschaft und Biochemie
- Ausgezeichnet als Lösungs-, Dispergiermittel und Emulgator
- Geeignet für die DNA- & RNA-Extraktion, Säugetierzellkulturen und Proteinaufreinigung
Verpackung
Jedes Kit enthält 3 Produktmuster à 100 ml aus jeweils verschiedenen Chargen.
Angaben zur Herstellung
Sonstige Hinweise
Rechtliche Hinweise
Empfehlung
Vergleichbares Produkt
auch häufig zusammen mit diesem Produkt gekauft
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
Flammpunkt (°F)
527.0 °F - Pensky-Martens closed cup
Flammpunkt (°C)
275 °C - Pensky-Martens closed cup
Persönliche Schutzausrüstung
Eyeshields, Gloves
Hier finden Sie alle aktuellen Versionen:
Analysenzertifikate (COA)
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Artikel
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
Verfügbare Verfahren zur Fluoreszenz-in-situ-Hybridisierung (FISH), Reagenzien und Ausstattung.
Protokolle
This page shows how to solubilize membrane proteins with products from Cytiva.
Auf dieser Seite wird die Solubilisierung von Membranproteinen mit Produkten von Cytiva dargestellt.
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What is the storage temperature of Tween 20?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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What is the minimum dilution ratio of Tween20 in Exosome incubating buffer based on CMC level?
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This product is not tested for any specific application. The critical micelle concentration of TWEEN 20 has been reported as 60 milligrams per liter. Please see the link below to review a publication that may be helpful:
https://open.clemson.edu/cgi/viewcontent.cgi?article=5274&context=all_thesesHelpful?
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Please provide the information on product container and material for P1379-500ML TWEEN® 20
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This item is packaged in a 500 ML HDPE Bottle with a red PP cap w/ PTFE liner.
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You state 0.06 mM as the CMC for this product. What is the stock concentration (in Molar) for this product? Alternatively what is the % to reach that 0.06 mM / CMC concentration? Thank you
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The critical micelle concentration (CMC) reported on the product page is a value reported from the literature. This product is a neat or pure liquid. The base molecular weight of this material is 1,225 daltons and the density is 1.095 g/mL. However, this is based on the assumption of 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid. Please see the link below to review the general Tween 20 datasheet, note that the CMC value listed is 60 mg/L.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/128/958/p9416pis.pdfHelpful?
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What is the grade/agency of Tween 20?
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This product is a research grade TWEEN 20.
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What is the expiry date once opened?
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Long-term stability studies have not been performed, however, providing that the product is stored and handled properly, the expected shelf life is not expected to change. Factors such as improper closure and cross-contamination would certainly impact the overall stability and shelf life.
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The data sheet for my lot says this is 50% pure. Does that mean it is 50% tween 20?
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The 50% is referring to the concentration of lauric acid which occurs in the highest concentration. Fatty acid constituents of this product are determined by transesterification to yield fatty acid methyl esters, which are identified by gas chromatography. Fatty acids that are typically present in the offered Tween products are not a quantitative measurement. These include myristic, palmitic, and stearic acid, for the most part. While the Tween is considered a 'pure' product, the molecular weight is calculated on the assumption of 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid.
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Can solutions of Product P1379, Tween® 20, be autoclaved?
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Increased temperature can lead to autoxidation of TWEEN 20 in solution. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. TWEEN 20 is heat sensitive; it will darken when exposed to elevated temperatures. Autoclaving is done at the customer's risk.
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