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Merck

318752

Sigma-Aldrich

Bromthymolblau Natriumsalz -Lösung

0.04 wt. % in H2O

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About This Item

Empirische Formel (Hill-System):
C27H27Br2NaO5S
CAS-Nummer:
Molekulargewicht:
646.36
MDL-Nummer:
UNSPSC-Code:
12171500
PubChem Substanz-ID:
NACRES:
NA.47

Form

liquid

Qualitätsniveau

Konzentration

0.04 wt. % in H2O

pH-Wert

6.0-7.6, yellow to blue

Dichte

0.99 g/mL at 25 °C (lit.)

λmax

615 nm

ε (Extinktionskoeffizient)

≥13000 at 304-310 nm in 0.1 M NaOH at 0.005 g/L
≥8000 at 391-397 nm in 0.1 M NaOH at 0.005 g/L

Anwendung(en)

diagnostic assay manufacturing
hematology
histology

Lagertemp.

room temp

SMILES String

[Na+].CC(C)c1cc(c(C)c(Br)c1O)\C(=C2/C=C(C(C)C)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O

InChI

1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;

InChIKey

NMKFVGALBGZKGW-FKWCIMQXSA-M

Allgemeine Beschreibung

Bromothymol Blue is a sulfonated hydroxyquinone in acidic solutions. In alkaline solutions, it is present as a dianion that is blue with a larger conjugated system than the yellow hydroxyquinone. Both forms are lipophilic. It is also known as bromthymol blue or dibromothymolsulfonphthalein.

Anwendung

Bromothymol blue has various applications in biology and medicine. It is suitable for demonstrating fungal hyphae within plant roots. It is used as a vital stain to trace the movement of fluids from the lymph. It is added to an agar gel microbial culture medium for enumeration of Bacillus cereus. Bromothymol blue is also used as a pH indicator due to its precise color transition properties at neutrality.

Biochem./physiol. Wirkung

Bromothymol Blue is a pH indicator which changes color from yellow (pH 6.0) to blue (7.6). It belongs to the class of sulfonephthalein dyes.

Lagerklassenschlüssel

10 - Combustible liquids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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