Product TMS-010 is a standard pH 2.5 solution of Alcian Blue that stains a number of substances, including chondrocytes, at this pH. The provided procedure is similar but not identical to the standard histology stain procedure found in most histology textbooks.
TMS-010
Alcian-Blue Staining Solution
Synonym(e):
Alcian blue 8G
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Größe auswählen
100 ML
CHF 179.00
CHF 179.00
Voraussichtliches Versanddatum02. Juni 2025
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100 ML
CHF 179.00
About This Item
UNSPSC-Code:
12352207
NACRES:
NA.75
CHF 179.00
Voraussichtliches Versanddatum02. Juni 2025
Empfohlene Produkte
Methode(n)
cell based assay: suitable
cell culture | stem cell: suitable
cell differentiation: suitable
Qualitätsniveau
Verwandte Kategorien
Allgemeine Beschreibung
Alcian blues, are a family of polyvalent basic dyes, of which, the Alcian blue 8G has been historically the most common and the most reliable member. It is used to stain acidic polysaccharides such as glycosaminoglycans in cartilages and other body structures, some types of mucopolysaccharides, sialylated glycocalyx of cells etc. Alcian blue if often used to identify Chondrocytes from differentiated cultures of both human and rodent mesenchymal stem cells.
Anwendung
Research Category
Stammzellforschung
Stammzellforschung
Research Sub Category
Osteobiologie
Osteobiologie
Qualität
Solution is blue with no particulates present. Solution is suitable for endpoint cell culture assays.
Physikalische Form
Product is presented as a 1% w/v Alcian Blue solution in acetic acid at pH 2.5.
Product is presented as a 1% w/v Alcian Blue solution.
Lagerung und Haltbarkeit
Store at room temperature for up to 4 months from date of receipt. If Alcian-Blue solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A
Lagerklassenschlüssel
3 - Flammable liquids
WGK
WGK 2
Flammpunkt (°F)
102.2 °F - closed cup
Flammpunkt (°C)
39 °C - closed cup
Analysenzertifikate (COA)
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Artikel
Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.
Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.
Protokolle
Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.
Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.
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What is the composition of the differentiation for TMS 010 C procedure?
1 answer-
Helpful?
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What is the concentration of acetic acid specified for the Alcian Blue pH 2.5 TMS-010-C product?
1 answer-
The concentration of acetic acid specified for the Alcian Blue pH 2.5 TMS-010-C product is not provided. However, it is a classical textbook formulation typically consisting of 1% Alcian Blue in 3% glacial acetic acid.
Helpful?
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Is there a procedure available for using TMS-010, and in this context, would you anticipate the alcian blue to be present in the nuclei, cytoplasm, or the entire cell?
1 answer-
Depending on the preparation of Alcian Blue, the dye can be used to identify various substances. TMS-010 seems to have been formulated to stain chondrocytes, the cells that produce collagen. It's expected that the staining should be primarily in the cytoplasm. However, if the staining intensity is high, it might appear that the entire cell is stained.
Helpful?
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Can you provide a protocol for a colorimetric assay that uses Alcian Blue to compare sulfated proteoglycan content in adherent cancer cell lines?
1 answer-
The Alcian-Blue Staining Solution (cat. no. TMS-010-C) is a cell culture product from the cell culture portfolio, intended for cell-based assays, cell differentiation, and stem cell culture. For example, to identify chondrocytes from differentiated cultures of both human and rodent mesenchymal stem cells (MSCs).
This product is presented as a 1% w/v Alcian Blue solution in acetic acid at pH 2.5.
The Certificate of Analysis does not include any usage instructions and there is no separate product data sheet or user guide.
The following paper may be helpful for the staining of stem cells:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432525/Helpful?
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