ECM815
Osteogenesis Quantitation Kit
The Osteogenesis Quantitation Kit provides all the necessary reagents & a protocol to enable quantification of osteogenesis using a standard plate reader.
Synonym(e):
Bone Formation Kit, Osteogenesis Detection Kit
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
About This Item
UNSPSC-Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Empfohlene Produkte
Qualitätsniveau
Hersteller/Markenname
Chemicon®
Methode(n)
cell based assay: suitable
Nachweisverfahren
colorimetric
Versandbedingung
wet ice
Allgemeine Beschreibung
CHEMICON′s Osteogenesis Quantitation Kit provides all the necessary reagents and a protocol to enable quantification of osteogenesis using a standard plate reader. This product is useful for studying the effects of growth factors, drugs and toxic agents on bone formation and for screening candidate osteogenic pharmaceuticals. Also, studies examining the intracellular signaling pathways regulating osteoblast differentiation can be facilitated by use of this kit.
The kit includes Alizarin Red Solution, a staining solution which can be used to visually detect the presence of mineralization in bone tissue. Also included are reagents for extraction of Alizarin Red from stained cells, and a protocol for performing quantitative analysis of the extracted Alizarin Red. Enough reagents are provided to perform 48 quantitation assays on 24 well plates.
For Research Use Only. Not for use in diagnostic procedures.
The kit includes Alizarin Red Solution, a staining solution which can be used to visually detect the presence of mineralization in bone tissue. Also included are reagents for extraction of Alizarin Red from stained cells, and a protocol for performing quantitative analysis of the extracted Alizarin Red. Enough reagents are provided to perform 48 quantitation assays on 24 well plates.
For Research Use Only. Not for use in diagnostic procedures.
Anwendung
Research Category
Zellstruktur
Zellstruktur
Note: Kit componenet 10X ARS Dilution Buffer: Part No. 2004810
One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent is provided. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use.
One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent is provided. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use.
Komponenten
Alizarin Red S Stain Solution (1X): Part No. 2003999, One bottle containing 50 mL Alizarin Red S Solution. Store at 2-8°C.
10% Acetic Acid: Part No. 2004807, One bottle containing 20 mL of 10% Acetic Acid in deionized water. Store at 2-8°;C.
10% Ammonium Hydroxide: Part No. 2004809, One bottle containing 10 mL of 10% Ammonium Hydroxide in deionized water. Store at 2-8°C.
10X ARS Dilution Buffer: Part No. 2004810, One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use. 80% acetic acid is not included with the kit. Store at 2-8°C.
10% Acetic Acid: Part No. 2004807, One bottle containing 20 mL of 10% Acetic Acid in deionized water. Store at 2-8°;C.
10% Ammonium Hydroxide: Part No. 2004809, One bottle containing 10 mL of 10% Ammonium Hydroxide in deionized water. Store at 2-8°C.
10X ARS Dilution Buffer: Part No. 2004810, One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use. 80% acetic acid is not included with the kit. Store at 2-8°C.
Lagerung und Haltbarkeit
All components should be stored at 2 - 8ºC until expiration date printed on label.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
Zielorgane
Respiratory system
Lagerklassenschlüssel
8B - Non-combustible corrosive hazardous materials
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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