PMEF-CF
EmbryoMax® primäre embryonale Fibroblasten der Maus
Synonym(e):
MEF Feeder Cells, CF-1 PMEFs, CF-1 MEFs, MEFs, Mouse Feeder Cells
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biological source
mouse
Quality Segment
manufacturer/tradename
Specialty Media, EmbryoMax®
technique(s)
cell culture | stem cell: suitable
input
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type primary embryotic fibroblasts (PMEFs)
sample type: mouse embryonic stem cell(s)
shipped in
liquid nitrogen
storage temp.
-140 to -196°C
General description
Plattierung der aus MEF-Zellen gewonnenen Feederzellen
Verfahren:
1. Vor dem Auftauen der pMEF-Feederzellen die Platten/Kolben mit einer Gelatinelösung beschichten.
2. pMEF-Fläschchen in einem 37 °C warmen Wasserbad rasch auftauen und in ein 15 mL Röhrchen überführen (enthält bereits ein 10 mL warmes pMEF-Feederzellmedium). Röhrchen vorsichtig invertieren, um Fibroblasten zu verteilen, und bei 300 xg 4–5 Minuten zentrifugieren.
3. Überstand entfernen und das Zellpellet in ein warmes pMEF-Feederzellmedium resuspendieren.
4. Gelatinelösung von Platten/Kolben entfernen und die pMEF-Feederzellsuspension bei den unter Tabelle 4.1 des Maus-ES-Protokolls aufgeführten empfohlenen Dichten aliquotieren.
5. pMEF-Feederzellen bei 37 °C mit 5 % CO2 inkubieren. Die im Maus-ES-Protokoll dargestellten Abbildungen 4A, B und C als Hilfe zur Einschätzung der richtigen pMEF-Dichte und
des richtigen Aussehens anwenden. Die mit Gelatine beschichteten Platten können für 12–14 Tage verwendet werden.
Biochem/physiol Actions
Packaging
Preparation Note
Legal Information
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Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
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Protokolle
Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
Artikel
Mouse embryonic fibroblasts (MEFs) serve as a feeder layer for both mouse and human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPSCs).
The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.
Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling
Verwandter Inhalt
"As transgenic mouse core laboratories know well, mouse models are a powerful means of determining the function of mammalian genes in the context of whole organisms—especially since over 95% of the mouse genome is similar to the human genome. However, you may not know that: 1. Laboratory mouse strains are descended from ancestors in modern-day Pakistan. 2. The first inbred strain of lab mouse was created in 1909. It was named DBA for its coat color: dilute brown non-agouti. 3. There is a mutant mouse actually named“Bad Hair Day” (Bhrd). Someone get this little guy a stylist! 4. Male mice, when treated with anticancer drugs, have been shown to pass on DNA instability to their offspring. 5. The founder of Jackson Laboratories, C. C. Little, once had lunch with Walt Disney to request that an animated film be created linking Mickey Mouse and the laboratory mouse. The request was denied."
The cell culture protocols described in this instructional manual include the in vitro culture of murine ES cells using EmbryoMax products along with ESGRO® mLIF medium supplement, as well as feeder-free and serum-free ES cell culture using the ESGRO Complete™ line of products. Also included in this instructional manual are: methods for serum-free neuronal differentiation of mouse ES cells, iPS cell generation using STEMCCA™ lentivirus kits, cre-recombinase mediated excision of STEMCCA™ genes from iPS cells, derivation and rescue of new and existing ES cell lines using RESGRO™ Culture Medium and ESGRO Complete™ system.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| PMEF-CF | 04053252667404 |