This product does contain Lactate. Please see the full product formulation below. Quantities are in mg/L.
MR-121
EmbryoMax® KSOM Mäuseembryo-Medien
(1X), Liquid, with 1/2 Amino Acids & Phenol Red
Synonym(e):
K+ Simplex Optimised Medium (KSOM), KSOM Media
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About This Item
Empfohlene Produkte
Qualitätsniveau
Form
liquid
Hersteller/Markenname
Specialty Media
EmbryoMax®
Methode(n)
cell culture | embryo: suitable
cell culture | stem cell: suitable
Aufnahme
sample type: mouse embryo(s)
Lagerung und Haltbarkeit
Einmal aufgetaut, sollte das Produkt innerhalb von 2 Wochen verwendet werden.
Hinweis: Unser EmbryoMax flüssiges Mäuseembryo-Medium wird alle zwei Monate hergestellt. Für die regelmäßige Verwendung empfehlen wir einen Dauerauftrag, um Lieferrückstände zu vermeiden.
Rechtliche Hinweise
Haftungsausschluss
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Artikel
Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
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HI, DOES IT HAVE ANY LACTATE IN THE MEDIUM? IF SO, WHAT CONCENTRATION?
1 answer -
Is the KSOM (MR-121-D) sufficient for embryo culture to the blastocyst stage on its own? In the literature, papers mention using "KSOM supplemented with amino acids." I observed that MR-121-D indicates it has "half amino acids." Essentially, I am trying to understand if individuals are adding extra amino acids to this media, or if the 1/2 amino acids already included are what they are referring to when they say "supplemented with amino acids."
1 answer-
KSOM (MR-121-D) is indeed sufficient for embryo culture up to the blastocyst stage. It is a 1X liquid solution with 1/2 amino acids and Phenol Red. When scientists reference KSOM with 1/2 amino acids, that is the intended composition, and they do not add the remaining 1/2 amino acids.
For more detailed information and historical context, the article "A brief history of the development of the KSOM family of media" provides insights into the development and usage of KSOM. According to documented literature and the mentioned article, KSOM has been shown to support a high yield of mouse blastocysts capable of developing into fetuses after transfer to surrogate mothers. The medium was notably improved when supplemented with 19 natural amino acids, with the added amino acids present at half the concentration described by Eagle. Dose response studies indicated little difference in the response between full strength and half strength concentrations of the amino acids.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790120/
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