MABN1847

Anti-Synapsin-1 Antibody, clone MEGS3-10A

clone MEGS3-10A, from mouse

• Synonym(e): Synapsin-1, Synapsin I
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: MEGS3-10A, monoclonal
• Speziesreaktivität: mouse
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• Isotyp: IgMκ
• NCBI-Hinterlegungsnummer: NP_038708.3
• UniProt-Hinterlegungsnummer: O88935
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Syn1 (20964)

Beschreibung

Allgemeine Beschreibung

Synapsin-1 (UniProt O88935; also known as Synapsin I) is encoded by the Syn1 (also known as Syn-1) gene (Gene ID 20964) in murine species. The synapins constitute a family of abundant neuronal phosphoproteins that regulate SV trafficking and neurotransmitter release at the pre-synaptic terminal. Three genes exisit in mammals, encoding altogether 11 synapsin members by alternative splicings (Synapsin Ia & Ib by SYN1, Synapsin IIa & IIb by SYN2, Synapsin IIIa through IIIf by SYN3), Syn III is the most precociously expressed isoform that has a role in the early phases of neural development and is downregulated in mature neurons. On the other hand, Syn I and Syn II are expressed at low levels at birth and their expressions progressively increase during synaptogenesis to reach a stable plateau at 1–2 months of life. The NH2-terminal region is divided in A, B, and C domains and is highly conserved among synapsin isoforms. Synapsins are regulated by postransloational phosphorylations. Domain A contains PKA and CaMKI/IV phosphorylation sites, domain B contains MAPK/Erk phosphorylation sites, and domain C is phosphorylated by Src. The c-terminal regions contain spliced domains and diverge among synapsin isoforms (domain D in Syn Ia and Ib, domain G in Syn IIa and IIb, domain H in Syn IIa, and domain J in Syn IIIa), although they all bear proline-rich regions binding to several SH3-containing proteins and additional phosphorylation sites for CaMKII, MAPK/Erk, and cdk1/5.

Immunogen

Recombinant full-length mouse synapsin-1.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Neurotransmitter & Rezeptoren

Immunocytochemistry Analysis: The ascites prior to antibody purification detected Synapsin-1 in primary mouse cortical neurons (Courtesy of Professor Michael E. Greenberg, Harvard Medical School, Boston, MA).
Western Blotting Analysis: An 1:1000 dilution of the ascites prior to antibody purification detected a higher Synapsin-1 expression in brain lysate from a 60-day old (P60) mouse than from a 6-day old (P6) mouse (Courtesy of Professor Michael E. Greenberg, Harvard Medical School, Boston, MA).

Qualität

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Synapsin-1 in 10 µg of mouse brain tissue lysate.

Zielbeschreibung

~75 kDa observed.

Physikalische Form

Format: Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable