MAB8152-I

Anti-West Nile Virus/Kunjin Antibody, NS1 Antibody, clone 3.1112G

• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 3.1112G, monoclonal
• Mol-Gew.: calculated mol wt 381.37 kDa
• Speziesreaktivität: virus
• Verpackung: antibody small pack of 100 μL
• Methode(n): ELISA: suitable; affinity binding assay: suitable; immunofluorescence: suitable; western blot: suitable
• Isotyp: IgM
• Epitopsequenz: Extracellular domain
• Protein-ID-Hinterlegungsnummer: NP_776015.1
• UniProt-Hinterlegungsnummer: P14335
• Versandbedingung: 2-8°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: vaccinia virus ... poly> POLY(912267)

Beschreibung

Allgemeine Beschreibung

Genome polyprotein (UniProt: P14335) is encoded by the POLY gene (Gene ID: 912267) in West Nile virus. West Nile virus (WNV) is a mosquito-borne positive-sense RNA virus of the Flavivirus genus within the Flaviviridae family. WNV genome encodes for three structural domains known as C, prM, and E and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). These are translated as a single polypeptide, which is subsequently cleaved by viral and cellular proteases. NS1 (aa 792-1143) is a glycoprotein that is involved in immune evasion, pathogenesis, and viral replication. It is secreted from infected cells and may vary its oligomeric state between monomers, dimers (the primary form) and hexamers. Once cleaved off the polyprotein, it is targeted to three destinations: the viral replication cycle, the plasma membrane, and the extracellular compartment. Intracellular NS1 functions as an essential cofactor for viral replication, and it localizes to WNV replication sites, whilst cell surface and secreted NS1 act as immunomodulators. NS1 is required for formation of the replication complex and recruitment of other non-structural proteins to the ER-derived membrane structures. It is reported to antagonize the complement function and inhibit signal transduction originating from Toll-like receptor 3. (Ref.: Martín-Acebes MA, et al. (2012). World J Virol. 1(2); 51-70).

Spezifität

Clone 3.1112G is a mouse monoclonal antibody that detects West Nile virus NS1 protein.

Immunogen

West Nile Virus (WNV; subtype KUNV) (isolate OR393).

Anwendung

Quality Control Testing

Evaluated by ELISA with recombinant West Nile Virus NS1 protein.

ELISA Analysis: Various concentrations of this antibody detected recombinant West Nile virus NS1 protein.

Tested Applications

ELISA Analysis: A representative lot detected West Nile Virus/Kunjin, NS1 in ELISA applications (Shelite, T.R., et al. (2008). Vector Borne Zoonotic Dis.;8(3):321-9; Ledermann, J.P., et al. (2011). Clin Vaccine Immunol.;18(4):580-7; Thompson, N.N., et al. (2012). Vector Borne Zoonotic Dis.;12(11):969-78).

Affinity Binding Assay: A representative lot of this antibody bound West Nile Virus/Kunjin with a KD of 1.0 x 10-12 in an affinity binding assay.

Western Blotting Analysis: A representative lot detected West Nile Virus/Kunjin, NS1 in Western Blotting applications (Whiteman, M.C., et al. (2011). Vaccine.;29(52):9702-10).

Immunofluorescence Analysis: A representative lot detected West Nile Virus/Kunjin, NS1 in Immunofluorescence applications (Garcia-Tapa, D. et al. (2006). Vet Immunol Immunopathol.;110(3-4):229-44; Giel-Moloney, M., et al. (2019). Sci Rep.;9(1):20005).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-West Nile Virus/Kunjin, NS1, clone 3.1112G, Cat. No. MAB8152-I, is a mouse monoclonal antibody that detects NS1 protein of West Nile virus and is tested for use in Affinity Binding Assay, ELISA, Immunofluorescence, and Western Blotting.

Physikalische Form

Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.

Lagerung und Haltbarkeit

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable