Direkt zum Inhalt
Merck
Alle Fotos(1)

Key Documents

MAB1330

Sigma-Aldrich

Anti-Collagen Type II Antibody, clone COLL-II

ascites fluid, clone COLL-II, Chemicon®

Synonym(e):

Anti-ANFH, Anti-AOM, Anti-COL11A3, Anti-SEDC, Anti-STL1

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

ascites fluid

Antikörper-Produkttyp

primary antibodies

Klon

COLL-II, monoclonal

Speziesreaktivität

sheep, human, bovine, rat, pig, mouse, canine

Hersteller/Markenname

Chemicon®

Methode(n)

ELISA: suitable
immunohistochemistry: suitable

Isotyp

IgG1

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... COL2A1(1280)

Spezifität

Reacts with both pepsin-solubilized and CNBr-cleaved human and bovine collagen type II. No cross-reactivity with collagen types I, III, V and IX.

Immunogen

Human (cartilage specific) CNBr-cleaved collagen II.

Anwendung

Research Category
Zellstruktur
Research Sub Category
ECM-Proteine
Immunofluorescence: 1:2-1:5 with fresh, frozen or acetone-fixed material. Mild pepsin digestion is recommended to enhance staining.

Western Blot: 1:1000-1:2000 with chemiluminescent detection

ELISA at 1:100-1:500, pepsin treated samples.





Optimal working dilutions must be determined by the end user.
This Anti-Collagen Type II Antibody, clone COLL-II is validated for use in ELISA, IH for the detection of Collagen Type II.

Physikalische Form

Unpurified
Ascites fluid containing no preservatives.

Lagerung und Haltbarkeit

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hinweis zur Analyse

Control
Cartilage in lung or fetus

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sie haben nicht das passende Produkt gefunden?  

Probieren Sie unser Produkt-Auswahlhilfe. aus.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Xin Lu et al.
International journal of molecular medicine, 37(2), 509-516 (2015-12-29)
The aim of the present study was to examine the expression levels and role of hsa-miR-15a in osteoarthritis (OA), as well as the associated mechanisms. The expression levels of hsa-miR-15a and A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type
Frederic Cailotto et al.
The Journal of biological chemistry, 285(14), 10572-10582 (2010-02-06)
The differentiated phenotype of chondrocyte is lost in pathological situations and after interleukin (IL)-1beta challenge. Wnt proteins and the inorganic pyrophosphate (PP(i)) transporter Ank regulate the differentiation process in many cell types. We investigated the possible contribution of Ank and/or
Yang-Jun Lao et al.
Orthopaedic surgery, 10(1), 56-63 (2018-02-13)
To investigate the effect of accumulated spinal axial biomechanical loading on mice lumbar disc and the feasibility of applying this method to establish a mice intervertebral disc degeneration model using a custom-made hot plate cage. In previous studies, we observed
Sarvpreet Singh et al.
Journal of cellular and molecular medicine, 16(4), 877-887 (2011-07-19)
Irradiation impacts on the viability and differentiation capacity of tissue-borne mesenchymal stem cells (MSC), which play a pivotal role in bone regeneration. As a consequence of radiotherapy, bones may develop osteoradionecrosis. When irradiating human bone-derived MSC in vitro with increasing
L A Vonk et al.
Osteoarthritis and cartilage, 22(1), 145-153 (2013-11-26)
Hsa-miR-148a expression is decreased in Osteoarthritis (OA) cartilage, but its functional role in cartilage has never been studied. Therefore, our aim was to investigate the effects of overexpressing hsa-miR-148a on cartilage metabolism of OA chondrocytes. OA chondrocytes were transfected with

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.