HTS086RTA
Ready-to-Assay H2 Histamine receptor Frozen Cells
Human
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About This Item
UNSPSC-Code:
41106514
eCl@ss:
32011203
NACRES:
NA.76
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Produktbezeichnung
Ready-to-Assay H2 Histamine receptor Frozen Cells,
Biologische Quelle
human
Qualitätsniveau
Hersteller/Markenname
Ready-to-Assay
Methode(n)
calcium flux assay: suitable
NCBI-Hinterlegungsnummer
Allgemeine Beschreibung
Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells, resuspends them in media, and allows them to recover for 24 hours. The following day the cells are counted, plated, and assayed for calcium response.
The histamine H2 receptor is a widely distributed G-protein coupled receptor, which is important in regulating a host of physiologic actions extending from gastric acid secretion to gastrointestinal motility (Del Valle and Gantz, 1997). Histamine mediated activation of its receptor leads to signaling through both adenylate cyclase and phosphoinositide /protein kinase C second messenger systems (Hill, 1990). Antagonists for this receptor have proven to be effective therapy for acid peptic disorders of the GI tract. Certain antagonists are used in the treatment of neuropsychiatric and neurological diseases e.g. schizophrenia, Alzheimer′s disease and Parkinson′s disease. Millipore′s cloned human H2-expressing cell line is made in the Chem-2 host, which supports high levels of recombinant H2 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at H2.
The histamine H2 receptor is a widely distributed G-protein coupled receptor, which is important in regulating a host of physiologic actions extending from gastric acid secretion to gastrointestinal motility (Del Valle and Gantz, 1997). Histamine mediated activation of its receptor leads to signaling through both adenylate cyclase and phosphoinositide /protein kinase C second messenger systems (Hill, 1990). Antagonists for this receptor have proven to be effective therapy for acid peptic disorders of the GI tract. Certain antagonists are used in the treatment of neuropsychiatric and neurological diseases e.g. schizophrenia, Alzheimer′s disease and Parkinson′s disease. Millipore′s cloned human H2-expressing cell line is made in the Chem-2 host, which supports high levels of recombinant H2 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at H2.
Beschreibung der Zelllinie
- GPCR Cell Lines
- Host cells: Chem-2
Anwendung
Human H2 GPCR frozen cells for Calcium Flux FLIPR Assays.
Biochem./physiol. Wirkung
- GPCR Class: A
- Protein Target: H2
- Target Sub-Family: Histamine
Komponenten
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
Haftungsausschluss
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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