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AB5519

Sigma-Aldrich

Anti-Prodynorphin Antibody

Chemicon®, from guinea pig

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

guinea pig

Qualitätsniveau

100

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Aufgereinigt durch

affinity chromatography

Speziesreaktivität

rat

Hersteller/Markenname

Chemicon®

Methode(n)

immunohistochemistry: suitable

NCBI-Hinterlegungsnummer

NM_024411.2

UniProt-Hinterlegungsnummer

P01213

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... PDYN(5173)

Spezifität

ProDynorphin, rat.

Immunogen

Synthetic peptide from rat ProDynorphin.

Anwendung

Anti-Prodynorphin Antibody is an antibody against Prodynorphin for use in IH.
Immunohistochemistry: 1:500

Optimal working dilutions must be determined by the end user.

APPLICATION NOTES FOR AB5519

IMMUNOHISTOCHEMISTRY

Perfusion: 1) Ca2+-free Tyrode′s solution followed by 2) paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.

Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5519. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

Physikalische Form

Affinity purified Guinea pig antibody in PBS containing 0.05% sodium azide.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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