567370

Smac-N7 Peptide

A peptide that contains the N-terminal seven residues of Smac (Second Mitochondria-derived Activator of Caspases, also known as DIABLO) and promotes procaspase-3 activation at around 10 µM.

• Synonym(e): Smac-N7 Peptide, H-AVPIAQK-OH
• Empirische Formel (Hill-System): C₃₃H₅₉N₉O₉
• Molekulargewicht: 725.88
• UNSPSC-Code: 12352200

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥95% (HPLC)
• Form: lyophilized solid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Farbe: white
• Löslichkeit: water: 1 mg/mL; DMSO: 5 mg/mL
• Versandbedingung: ambient
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

A peptide that contains the N-terminal seven residues of Smac (Second Mitochondria-derived Activator of Caspases, also known as DIABLO) and promotes procaspase-3 activation at around 10 µM. Reported to bind to the BIR2 and BIR3 (baculovirus IAP repeat) domains of XIAP (X-linked inhibitor of apoptosis proteins) with a K_d ~800 nM. Promotes the catalytic activity of caspase-9 in the presence of XIAP, suggesting that Smac competes with caspase-9 for binding to the same pocket on the surface of XIAP.

A peptide that contains the N-terminal seven residues of Smac (Second mitochondria-derived activator of caspases, also known as DIABLO) and promotes pro-caspase-3 activation at ~10 µM. Reported to bind to the BIR2 and BIR3 (baculovirus IAP repeat) domains of XIAP (X-linked inhibitor of apoptosis proteins) (K_d = ~800 nM). Promotes the catalytic activity of caspase-9 in the presence of XIAP, suggesting that Smac competes with caspase-9 for binding to the same pocket on the surface of XIAP.

Biochem./physiol. Wirkung

Cell permeable: no

Kd ~800 nM in binding to the BIR2 and BIR3 (baculovirus IAP repeat) domains of XIAP (X-linked inhibitor of apoptosis proteins)

Primary Target
Promotes the catalytic activity of caspase-9 in the presence of XIAP

Product does not compete with ATP.

Reversible: no

Verpackung

Packaged under inert gas

Warnhinweis

Toxicity: Standard Handling (A)

Sequenz

H-Ala-Val-Pro-Ile-Ala-Gln-Lys-OH

Physikalische Form

Supplied as a trifluoroacetate salt.

Rekonstituierung

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Sonstige Hinweise

Srinivasula, S.M., et al. 2001. Nature410, 112.
Chai, J., et al. 2000. Nature406, 855.
Liu, Z., et al. 2000. Nature408, 1004.
Srinivasula, S.M., et al. 2000. J. Biol. Chem.275, 36152.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable