1.24546

Fluorescein-5-isothiocyanate (FITC)

for biochemistry and for fluorescence microscopy

• Synonym(e): Fluorescein-5-isothiocyanate (FITC)
• Empirische Formel (Hill-System): C₂₁H₁₁NO₅S
• CAS-Nummer: 3326-32-7
• Molekulargewicht: 389.38
• MDL-Nummer: MFCD00005063
• UNSPSC-Code: 12171501
• EG-Index-Nummer: 222-042-0

Eigenschaften

• Qualitätsniveau: 400
• Form: solid
• Zusammensetzung: Dye, ≥95% spectrophotometric assay
• mp (Schmelzpunkt): >360 °C
• εmax: 2200-2380 at 488-494 nm in 0.1 M NaOH at 0.003 g/L
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: 2-8°C
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChIKey: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

In immuno histology/ cytology, Fluorescein-5-isothiocyanate (FITC) for biochemistry and for fluorescence microscopy, serve as marker substance for the visualization and for the detection of tissue-bound bio molecules like e.g. antigens, lectins, various proteins, peptides, nucleic acids, oligo- and polysaccharides in samples of human origin. FITC-marked antibodies bind specifically to the appropriate target structures in the tissue. An optimum FITC-protein (antibody) ratio is important for a good result.
FITC is an IVD registered product and CE certified, thus can be used for clinical diagnostic purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Hinweis zur Analyse

Farbstoffgehalt (spektralphotometrisch): ≥ 95 %
Identität (UV/VIS-Spektrum): entspricht
Absorptionsmaximum λ max. (Natronlauge 0.1 mol/l): 488 - 494 nm
Spez. Extinktion E1%/1cm (λ_max; 0.003 g/l; NaOH 0.1 mol/l): 2200 - 2380
DC-Prüfung: entspricht

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°C): Not applicable