1.15940

Crystal violet (C.I. 42555)

for microscopy Certistain^®

• Synonym(e): Crystal violet (C.I. 42555), Hexamethylenepararosaniline chloride, Gentian violet (C.I. 42555), Hexamethyl-p-rosanilinium, Methyl violet 10 B
• Empirische Formel (Hill-System): C₂₅H₃₀ClN₃
• CAS-Nummer: 548-62-9
• Molekulargewicht: 407.98
• MDL-Nummer: MFCD00011750
• UNSPSC-Code: 41116130
• EG-Index-Nummer: 208-953-6

Eigenschaften

• Qualitätsniveau: 400
• Form: solid
• Wirksamkeit: 420 mg/kg LD₅₀, oral (Rat)
• Zusammensetzung: Dye, ≥88% spectrophotometric assay
• pH-Wert: 2.5-3.5 (20 °C, 10 g/L in H₂O)
• mp (Schmelzpunkt): 189-194 °C
• Löslichkeit: 10 g/L
• Dichte: 1.19 g/cm³ at 20 °C
• Schüttdichte: 220‑400 kg/m³
• εmax: 2050-2450 at 589-594 nm in water at 0.002 g/L
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: 2-30°C
• InChI: 1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
• InChIKey: ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Beschreibung

Allgemeine Beschreibung

Crystal violet (C.I. 42555) for microscopy Certistain^®, is a dry dye that can be used to produce a Crystal violet staining solution to differentiate Gram-positive and Gram-negative bacteria in samples of human origin. In the case of Gram-positive bacteria, the bacteria retain their dark blue / dark purple color even after the treatment with decolorizing solution. On the other side Gram-negative bacteria are stained red or pink with the counterstain. The Certistain^® dry dye can be used for clinical diagnostic purposes as it is certified and registered as IVD and CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Hinweis zur Analyse

Farbstoffgehalt (spektralphotometrisch): ≥ 88 %
Identität (UV/VIS-Spektrum): entspricht
Absorptionsmaximum λ max. (Wasser): 589 - 594 nm
Spez. Extinktion E1%/1cm (λ_max; 0.002 g/l; Wasser): 2050 - 2450
DC-Prüfung: entspricht
Michlers Keton: < 0.10 %
Trocknungsverlust (110 °C): ≤ 10 %
Eignung für die Mikroskopie: entspricht

Rechtliche Hinweise

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS05,GHS07,GHS08,GHS09
• Signalwort: Danger
• H-Sätze: H302 - H318 - H351 - H410
• P-Sätze: P201 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Gefahreneinstufungen: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3