330002S

E06 mAb-biotinylated

E06 Mouse Monoclonal Antibody (IgM), Biotinylated Anti-(Oxidized Phospholipid), 1X TBS (Tris Buffered Saline) with 1%BSA

• Synonym(e): E06 mAb-biotinylated, T15 antibody
• UNSPSC-Code: 12352203
• NACRES: NA.45
• citations: 3

Eigenschaften

• Antikörperform: purified from hybridoma cell culture
• Antikörper-Produkttyp: primary antibodies
• Assay: >95%
• Form: solution
• Verpackung: pkg of 1 × 0.125 mL (polypropylene tube with screw cap (330002S-25ug))
• Hersteller/Markenname: Avanti Research^™ - A Croda Brand 330002S
• Konzentration: 0.2 mg/mL (330002S-25ug)
• Versandbedingung: dry ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Avanti now provides high purity E06 from an animal-free culture of hybridoma cells. The E06 antibody was developed by Dr. Joseph Witztum at the University of California, San Diego using apolipoprotein E knock-out mice fed a high-fat diet for seven months.

Anwendung

E06 mAb-biotinylated has been used in immunodetection.[1] It may be used in immunohistochemistry,[2] western blot analysis and in in vivo imaging with magnetic resonance imaging (MRI) techniques.

The E06-Biotin is primarily used for sandwich ELISA determination of ox-LDL in serum or plasma samples. It can also be used in direct ELISA, competitive (IHC) and Western Blot analysis. For use in direct ELISA, make a 4 μg/mL working solution of 12 μg E06-Biotin in 3 mL of 1% BSA in PBS (1:62.5 dilution). Make a serial (1:1) dilution of this working solution to titrate the antibody. For competitive ELISA make a 2.5 μg/mL working solution (1:100 dilution).

Biochem./physiol. Wirkung

The E06 antibody is a murine monoclonal IgM natural antibody that binds to oxidized phospholipid and reacts with oxidized Low-density lipoprotein (LDL),[3] oxidized High-density lipoprotein (HDL) and protein covalently modified by oxidized phospholipid. This antibody has been used to probe atherosclerotic plaque in rabbits, monkeys,[2] mice, zebrafish and humans. It also binds to apoptotic cells, but not viable cells.

Verpackung

0.5 mL Polypropylene Tube with Screw Cap (330002S-25ug)

Rechtliche Hinweise

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable