ZRB1913

Anti-ATF6α Antibody, clone 1F11 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : ATF6-alpha, Activating transcription factor 6 alpha, Cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1F11, recombinant monoclonal
• Description: 1F11 Clone
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 74.59 kDa; observed mol wt ~65 kDa
• Produit purifié par: using Protein A
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): monkey
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• Technique(s): ELISA: suitable; affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: N-terminal half
• Numéro d'accès Protein ID: NP_031374
• Numéro d'accès UniProt: P18850
• Autre catégorie plus écologique: Aligned
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Informations sur le gène: human ... ATF6(22926)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1F11 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects ATF6 . It targets an epitope within 25 amino acids from the N-terminal half.

Immunogène

KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal half of human ATF6 .

Application

Quality Control Testing

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected ATF6 in MCF-7 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected ATF6 in lysates from HeLa and HEK293 cells.

Affinity Binding Assay: A representative lot of this antibody bound ATF6 peptide with a KD of 2.0 x 10-7 in an affinity binding assay.

Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected GST-tagged recombinant fragment of Human ATF6 .

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ATF6 in human placenta tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Cyclic AMP-dependent transcription factor ATF-6 alpha (UniProt: P18850; also known as cAMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, ATF6-alpha) is encoded by the ATF6 gene (Gene ID: 22926) in human. ATF6 is an endoplasmic reticulum (ER)-localized protein that is involved in transducing signals linked to ER stress. It is responsible for the correct folding, secretion, and membrane insertion of a vast majority of proteins in eukaryotic cells. Two homologous ATF6 proteins, ATF6α and ATF6β, are expressed in mammalian cells. Their C-terminal region protrudes into the ER lumen, whereas the N-terminal faces the cytosol. Although ATF6α and ATF6β display significant sequence homology, they exhibit divergent transcriptional activation domains.  ATF6α is a single-pass type II membrane protein with a cytoplasmic domain (aa 1-377), a transmembrane domain (aa 378-398), and a lumenal domain (aa 399-670). It is a homodimeric protein that can form heterodimer with ATF6β. This dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). ATF6α is a potent transcriptional activator whereas ATF6β is a poor transcriptional activator, and may even inhibit activation by ATF6α. ER stress is known to promote its processing that releases N-terminal transcription factor form that translocates into the nucleus, where it activates transcription of genes involved in unfolded protein response (UPR). Mutations in ATF6 gene are known to cause Achromatopsia 7 (ACHM7), an ocular stationary disorder due to the absence of functioning cone photoreceptors in the retina. It is characterized by total colorblindness, low visual acuity, photophobia, and nystagmus. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hillary, RF., and FitzGerald, U. (2018). J. Biomed. Sci. 25; Article 48; Thuerauf , DJ., et al. (2007). J. Biol. Chem. 282(31); 22865-22878; Yoshida, H., et al. (2001). Mol. Biol. Cell. 21(4); 1239-1248; Haze, K., et al. (1999). Mol. Biol. Cell. 10(11); 3787-3799).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable