R0884

T7 RNA Polymerase

recombinant, expressed in E. coli, buffered aqueous solution

• Synonyme(s) : RNA Polymerase T7, RNA Polymerase, T7 from E. coli HMS 174/pAR1219
• Numéro CAS: 9014-24-8
• Numéro de classification (Commission des enzymes): 2.7.7.6 (BRENDA, IUBMB)
• Numéro MDL: MFCD00132197
• Code UNSPSC : 12352204

Propriétés

• Produit recombinant: expressed in E. coli
• Qualité: for molecular biology
• Forme: buffered aqueous solution
• Poids mol.: 98.8 kDa
• Concentration: 10,000-50,000 U/mL
• Numéro d'accès UniProt: P00573
• Activité étrangère: DNase and RNase, none detected
• Température de stockage: −20°C
• Informations sur le gène: bacteriophage T7 ... T7p07(1261050)

Description

Description générale

T7 RNA polymerase is highly specific for the bacteriophage T7 promoter and terminator sequences. It is extensively used to prepare RNA transcripts for stuctural and metabolic studies. The RNA transcripts can be converted to probes for sensitive hybridization detection studies. T7 polymerase and dideoxynucleotides can be used to directly sequence DNA.

Composants

T7 RNA Polymerase is supplied as a solution of 100 mM NaCl, 50 mM Tris-HCl (pH 7.9), 0.1 mM EDTA, 0.1% Triton X-100, 1 mM DTT, and 50% (v/v) glycerol.

Définition de l'unité

One unit will catalyze the incorporation of 1 nmol of rNTP into acid-precipitable material in 60 min at 37°C.

Remarque sur l'analyse

Activity assay: 40 mM Tris-HCl, pH 7.9, 6 mM MgCl₂, 4 mM spermidine, 10 mM DTT, 0.5 μM each rNTP + 10 μCi α-³²P-UTP, 3-10 units of enzyme, and 1 μg of a 350 bp template are incubated for 10 min at 37°C in a total volume of 100 μl. Typical results are ≥50% incorporation of labeled nucleotide into ≥90% full-length transcript.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable