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R1158

Sigma-Aldrich

Ribonuclease Inhibitor

recombinant, expressed in E. coli, 20-40 units/μL

Synonyme(s) :

RNAse inhibitor

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About This Item

Numéro MDL:
MFCD00132187
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.52

Produit recombinant

expressed in E. coli

Niveau de qualité

300

Qualité

for molecular biology

Forme

buffered aqueous solution

Poids mol.

~50 kDa

Concentration

20-40 units/μL

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Description générale

Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is derived from E. coli which expresses portions of the human placental ribonuclease inhibitor. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes. The pH range for inhibition is pH 5.5–9 (highestinhibition at pH 7–8).

Application

Ribonuclease Inhibitor is suitable for use in useful for in vitro inhibition of ribonucleases, including procedures like:

  • cDNA synthesis from mRNA
  • reverse transcriptase polymerase chain reaction (RT-PCR)
  • in vitro transcription and translation
  • mRNA protection from degradation
  • formaldehyde cross-linked lncRNA purification
  • RNA-fluorescence in situ hybridization (FISH)
Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.

Actions biochimiques/physiologiques

Inhibits RNase by forming a tight, non-covalent 1:1 complex.

Composants

Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.

Définition de l'unité

One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.

Forme physique

Solution in 20 mM HEPES-KOH, pH 7.6, 50 mM KCl, 8 mM DTT and 50% glycerol.

Autres remarques

  • Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause a release of active ribonuclease from the complex.
  • This product is for R&D use only, not for drug, household, or other uses.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Lintao Li et al.
Cell cycle (Georgetown, Tex.), 22(4), 476-493 (2022-11-13)
Whether long non-coding RNA Mir-99a-Let-7c Cluster Host Gene (LncRNA MIR99AHG) is involved in osteoporosis (OP) remains vague, so we hereby center on its implication. Old C57BL/6J mice were injected with the silencing lentivirus of MIR99AHG and subjected to microCT analysis
Antisense overlapping long non-coding RNA regulates coding arachidonate 12-lipoxygenase gene by translational interference
Sabbir MG, et al.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1866(10), 158987-158987 (2021)
A fine-needle aspiration-based protein signature discriminates benign from malignant breast lesions
Franzen B, et al.
Molecular Oncology, 12(9), 1415-1428 (2018)
Comparison of in vitro viability methods for Cryptosporidium oocysts
Burgt NHV, et al.
Experimental Parasitology, 187, 30-36 (2018)
Nathan H Vande Burgt et al.
Experimental parasitology, 187, 30-36 (2018-03-09)
The water-borne protozoan parasite Cryptosporidium parvum forms oocysts that can persist for long periods of time in the environment, even though the sporozoites inside the oocysts may no longer be viable, making it difficult to assess the associated risk of
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