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P3125

Sigma-Aldrich

Papaïne from papaya latex

buffered aqueous suspension, 2× Crystallized, ≥16 units/mg protein

Synonyme(s) :

Papaïnase

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About This Item

Numéro CAS:
Numéro de classification (Commission des enzymes):
Numéro MDL:
Code UNSPSC :
12352204
eCl@ss :
42030311
Nomenclature NACRES :
NA.54

Forme

buffered aqueous suspension

Qualité

2× Crystallized

Activité spécifique

≥16 units/mg protein

Poids mol.

23.4 kDa

Température de stockage

2-8°C

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Description générale

Papain, a cysteine protease, consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group.

Application

Papain from papaya latex has been used to digest:
  • Muller glial cells
  • cartilage explants
  • GAG and DNA content of the tissue constructs
  • decellularized tissue samples.

Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules.
Sert à produire des fragments Fab d′anticorps. Également utilisée pour la dissociation cellulaire, car des études ont montré qu′elle s′avère plus efficace et moins détériorante avec certains tissus.

Actions biochimiques/physiologiques

Papain has broad specificity, in cleaving peptide bonds of basic amino acids, leucinehydrolyzes esters and amides. Papain digests many protein substrates extensively than pancreatic proteases. Papain is commonly used in cell isolation procedures since it is more efficient and less harmful compared to other proteases. Papain significantly increases the yield of viable smooth muscle cells without affecting the cell′s sensitivity to stimulants. It catalyzes several reactions, like hydrolysis, transferase action, specificity and acyl-enzyme intermediate.
Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
Protéase à cystéine qui clive les liaisons peptidiques des acides aminés basiques, de la leucine ou de la glycine.
pH optimal situé entre 6,0 et 7,0.
Hydrolyse également les esters et les amides.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of N-α-benzoyl-L-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 °C.

Forme physique

Suspension in 0.05 M sodium acetate, pH 4.5, containing 0.01% thymol

Autres remarques

Pour en savoir plus sur la papaïne, rendez-vous sur www.sigma-aldrich.com/enzymeexplorer.

Substrat

Réf. du produit
Description
Tarif

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Les clients ont également consulté

Expression of non-visual opsins Opn3 and Opn5 in the developing inner retinal cells of birds. Light-responses in Muller glial cells
Rios MN, et al.
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Scanningless and continuous 3D bioprinting of human tissues with decellularized extracellular matrix
Yu C, et al.
Biomaterials, 194, 1-13 (2019)
Research applications of proteolytic enzymes in molecular biology
Motyan J, et al.
Biomolecules, 3(4), 923-942 (2013)
The dog as a model for osteoarthritis: the FGF4 retrogene insertion may matter
Tellegen AR, et al.
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society (2019)
The structure of papain
Advances in Protein Chemistry, 25, 79-115 (1971)

Articles

Antibody fragmentation with our pepsin digestion protocol for IgG antibody fragmentation and preparation of F(ab’).

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.

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