P3076

Platelet-Derived Growth Factor-AA human

≥98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonyme(s) : PDGF−AA
• Numéro MDL: MFCD00081862
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• product name: Platelet-Derived Growth Factor-AA human, PDGF-AA, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli
• Pureté: ≥98% (SDS-PAGE and HPLC)
• Forme: lyophilized powder
• Puissance: 1.0-3.0 ng/mL ED₅₀
• Qualité: endotoxin tested
• Poids mol.: 28.5 kDa (containing 250 amino acid residues)
• Conditionnement: pkg of 10 μg; pkg of 50 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <1 EU/μg
• Numéro d'accès UniProt: P04085
• Température de stockage: −20°C
• Informations sur le gène: human ... PDGFRA(5154)

Description

Description générale

Platelet-derived growth factor (PDGF) is composed two polypeptide subunits of A and B chain. PDGF is produced by a number of cells including smooth muscle cells, endothelial cells and macrophages. The PDGF gene is highly conserved and is mapped to human chromosome 7p22.3. PDGF is structurally and functionally related to vascular endothelial growth factors.

Actions biochimiques/physiologiques

Cell division and relocalization of pulmonary artery smooth muscle cells and fibroblasts is known to be triggered by platelet-derived growth factor (PDGF). Due to its proliferatory function, it is associated with fibroproliferative disorders such as atherosclerosis, pulmonary arterial hypertension and lung fibrosis. PDGF exists both in homo and heterodimeric forms and stimulates α and β-cell surface receptors. Vascular endothelial growth factor expression is regulated by PDGF-AA autocrine signalling and is known to promote tumor angiogenesis in vivo.

Autres remarques

Homodimer of the A chain of human PDGF.

Forme physique

Sterile-filtered through a 0.2 μm filter. Lyophilized with 10 mM acetic acid.

Remarque sur l'analyse

The biological activity is measured by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable