P3201

Platelet-Derived Growth Factor-BB human

≥98% (HPLC and SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonyme(s) : PDGF-BB
• Numéro MDL: MFCD00213023
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• product name: Platelet-Derived Growth Factor-BB human, PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Pureté: ≥98% (HPLC and SDS-PAGE)
• Forme: lyophilized powder
• Puissance: 1.0-3.0 ng/mL EC₅₀
• Qualité: endotoxin tested
• Poids mol.: ~25 kDa
• Conditionnement: pkg of 10 μg; pkg of 50 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: ≤1 EU/μg
• Numéro d'accès UniProt: P01127
• Température de stockage: −20°C
• Informations sur le gène: human ... PDGFB(5155)

Description

Description générale

Platelet-derived growth factor (PDGF) is expressed as homo and heterodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD. The PDGF gene is highly conserved mapped to human chromosome 7p22.3. The active form of Pdgf exists as disulfide-linked dimers. It consists of cystine-knot-fold growth factor domain, which is highly conserved, except for the N-terminal prodomain, which shows variation in length among the isoforms.

Application

Platelet-Derived Growth Factor-BB human has been used :

• As a growth supplement in proximal tubule and glomerular explant cell culture
• In three-dimensional scaffold preparation
• To study the effect of brivanib on growth factor signaling pathways
• In transwell migration assay

Actions biochimiques/physiologiques

Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF significantly activates chemotaxis of neutrophils, monocytes, and fibroblasts. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment. PDGF-BB is an important regulator of bone homeostasis, repair and regeneration.

Autres remarques

Dimer of the B chain of human PDGF.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 30 mM acetic acid.

Remarque sur l'analyse

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)