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Key Documents

I4777

Sigma-Aldrich

Interferon-γ from mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonyme(s) :

IFN-γ

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About This Item

Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

mouse

Niveau de qualité

Produit recombinant

expressed in E. coli

Pureté

≥98% (SDS-PAGE)

Forme

lyophilized powder

Poids mol.

protein 15.6 kDa

Conditionnement

pkg of 0.1 mg

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Couleur

white

Solubilité

water: soluble 1.000 mL, clear, colorless

Numéro d'accès UniProt

Température de stockage

−20°C

Informations sur le gène

mouse ... Ifng(15978)

Application

Interferon-γ from mouse has been used:
  • for in vitro stimulation of the BMDC (bone marrow derived dendritic cell), BMM (bone marrow-derived macrophages) and CD11c+ (CD11 antigen-like family member C) lung macrophages
  • for stimulation of murine macrophages to induce the production of nitrite and nitrate
  • to check CCAAT/enhancer binding protein β (C/EBPβ)-mediated changes in the expression of prostaglandin synthesis enzymes in neuroinflammation

Actions biochimiques/physiologiques

Ifng (interferon-γ) plays an important role in innate and adaptive immune responses, and helps in defense against bacterial infections, dimorphic yeast and some viral infections.

Forme physique

Lyophilized from a 0.2 μm filtered buffered solution. Package size based on protein content.

Remarque sur l'analyse

The biological activity of recombinant mouse IFN-γ is measured by its ability to inhibit the proliferation of murine WEHI-279 cells.

Produit(s) apparenté(s)

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Sergio Arias-Negrete et al.
Analytical biochemistry, 328(1), 14-21 (2004-04-15)
Preparation of a nitrate reductase lysate of Escherichia coli MC1061 to measure nitrate and nitrite in biologic fluids is described. To obtain the crude bacterial lysate containing nitrate reductase activity, E. coli MC1061 was subjected to 16-20 freeze-thawing cycles, from
S N Vogel et al.
Infection and immunity, 38(2), 681-685 (1982-11-01)
Silica has been used for many years as an agent which selectively alters macrophage functions and, as such, has been used to assess the role of macrophages in the immune response to a variety of microbial and chemically defined agents.
P W Gray et al.
Proceedings of the National Academy of Sciences of the United States of America, 80(19), 5842-5846 (1983-10-01)
The murine immune interferon (IFN-gamma) gene was cloned and expressed under control of the simian virus 40 early promoter in the monkey COS-1 cell line. A protein is secreted from these cells having the biological, antigenic, and biochemical characteristics of
Shaoxi Yan et al.
Mediators of inflammation, 2021, 9976912-9976912 (2021-09-01)
Inflammation causes tissue damage and promotes ventricular remodeling after myocardial infarction (MI), and the infiltration and polarization of macrophages play an important role in regulating inflammation post-MI. Here, we investigated the anti-inflammatory function of curcumin after MI and studied its
Tatiana Cheusova et al.
Genes & development, 20(13), 1800-1816 (2006-07-05)
The release of Agrin by motoneurons activates the muscle-specific receptor tyrosine kinase (MuSK) as the main organizer of subsynaptic specializations at the neuromuscular junction. MuSK downstream signaling is largely undefined. Here we show that protein kinase CK2 interacts and colocalizes

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