H3662

HEPES sodium salt solution

BioReagent, 1M, suitable for cell culture

• Formule empirique (notation de Hill): C₈H₁₇N₂NaO₄S
• Numéro CAS: 75277-39-3
• Poids moléculaire : 260.29
• Numéro MDL: MFCD00036463
• Code UNSPSC : 12161700
• ID de substance PubChem : 24895590
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 200
• Stérilité: sterile-filtered (0.2 μm filtered)
• Gamme de produits: BioReagent
• Forme: solution
• Concentration: 0.90-1.10 M
• Technique(s): cell culture | mammalian: suitable
• Impuretés: Bioburden, tested; DNase, RNase. Protease, Nickase, free; endotoxin, tested; <5 ppm Heavy metals (as Pb)
• Traces de cations: Fe: <5 ppm
• Chaîne SMILES: [Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
• InChI: 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
• Clé InChI: RDZTWEVXRGYCFV-UHFFFAOYSA-M

Description

Description générale

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.

Application

HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)