324626

EGTA, Molecular Biology Grade

• Synonyme(s) : EGTA, Molecular Biology Grade, Ethyleneglycol- bis(β-aminoethyl)-N,N,Nʹ,Nʹ-tetraacetic Acid
• Formule empirique (notation de Hill) : C₁₄H₂₄N₂O₁₀
• Numéro CAS: 67-42-5
• Poids moléculaire : 380.35
• UNSPSC Code: 12352106
• NACRES: NA.25
• MDL number: MFCD00004291

Propriétés

• grade: Molecular Biology
• Quality Segment: 100
• description: RTECS - AH3760000
• assay: ≥98% dry basis (complexometric)
• form: solid (fine)
• reaction suitability: reagent type: chelator
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: 1 M NaOH: soluble
• cation traces: heavy metals (as Pb): ≤0.001%
• foreign activity: DNases, proteases, RNases, none detected
• shipped in: ambient
• storage temp.: 10-30°C
• SMILES string: N(CCOCCOCCN(CC(=O)O)CC(=O)O)(CC(=O)O)CC(=O)O
• InChI: 1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)
• InChI key: DEFVIWRASFVYLL-UHFFFAOYSA-N

Description

General description

Chelating agent selective for Ca²⁺. Binds one cation per molecule. Induces apoptosis in pheochromocytoma (PC12) cells.

Application

• Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.: This study presents a genetically encoded biosensor for real-time monitoring of lysosomal pH changes, potentially enhanced by buffering agents like EGTA. The findings may offer insights into cellular mechanisms influenced by calcium chelation (Ponsford et al., 2021).


• Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca(2+).: The research highlights the role of calcium in fungal development, with EGTA potentially influencing these biological processes through calcium chelation (Zhu et al., 2019).

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 year at 4°C.

Other Notes

Lindenboim, L., et al. 1995. J. Neurochem.64, 1054.
Bers, D.M. 1982. Am. J. Physiol.242, C404.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• Que se passe-t-il ?: WGK 2